Citation: XU Hong-Hui, XU Jing-Sha, HE Jun, PU Jing-Jiao, DU Rong-Guang, QI Bing. Detemination of Organic Tracers from Biomass Burning Emissions by High Performance Liquid Chromatography-Tandem Mass Spectrometry. Chinese Journal of Analytical Chemistry, 2018, 46(9): 1432-1437. doi: 10.11895/j.issn.0253-3820.170123 [复制]
Detemination of Organic Tracers from Biomass Burning Emissions by High Performance Liquid Chromatography-Tandem Mass Spectrometry
采用高效液相色谱和三重四极杆线性离子阱质谱联用，优化了相关分析参数，建立了3种目标有机示踪物左旋葡聚糖、甘露聚糖及半乳聚糖直接检测的快速分析方法。研究结果表明，在流动相氨水浓度为0.0005%（w/V）、流速0.35 mL/min、柱温箱温度45℃和负离子模式条件下，左旋葡聚糖、甘露聚糖及半乳聚糖的线性范围为0.01~1.00 mg/L（R2 > 0.999），检出限分别为1.8、5.8和9.5 μg/L。本方法可应用于外场采样点所采集的气溶胶样品的测试，实现了大气气溶胶中生物质有机示踪物的快速、准确、灵敏检测。
A fast analytical method for organic tracers from biomass burning emissions including levoglucosan, mannosan and galactosan was developed using high performance liquid chromatography (HPLC)-tandem mass spectrometry (MS/MS) without derivatization process. The optimum analytical conditions were established as follows:ammonium hydroxide (0.005%, w/V) as mobile phase with a flow rate of 0.35 mL/min, column temperature of 45℃, and negative ion electrospray ionization. Under the optimal analytical conditions, the linear concentration ranges of these organic tracers were 0.01-1 mg/L (R2 > 0.999) and limits of detection (LOD) were 1.8 μg/L for levoglucosan, 5.8 μg/L for mannosan and 9.5 μg/L for galactosan, respectively. This new method was validated and applied to the detection of real aerosol samples, and was proved to be accurate, fast and sensitive for analysis of organic tracers from biomass burning emissions.