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引用本文:   李好问, 华鑫, 龙亿涛. 金属/基质增强飞行时间-二次离子质谱用于单细胞脂质分析. 分析化学, 2018, 46(1): 61-66. doi:  10.11895/j.issn.0253-3820.171014 [复制]

Citation:   LI Hao-Wen, HUA Xin, LONG Yi-Tao. Metal/Matrix Enhanced Time-of-Flight Secondary Ion Mass Spectrometry for Single Cell Lipids Analysis. Chinese Journal of Analytical Chemistry, 2018, 46(1): 61-66. doi: 10.11895/j.issn.0253-3820.171014 [复制]

金属/基质增强飞行时间-二次离子质谱用于单细胞脂质分析

通讯作者:  华鑫, xinhua@ecust.edu.cn

收稿日期: 2017-06-16

接受日期: 2017-10-05

出版日期: 2018-01-20

基金项目: 本文系上海市青年科技英才扬帆计划(No.17YF1403000)、上海市自然科学基金(No.17ZR1407700)和中国博士后科学基金(No.2017M611470)资助

Metal/Matrix Enhanced Time-of-Flight Secondary Ion Mass Spectrometry for Single Cell Lipids Analysis

Corresponding author:  HUA Xin, xinhua@ecust.edu.cn

Received Date:  2017-06-16

Accepted Date:  2017-10-05

Published Date:  2018-01-20

单细胞中化学成分的分析对细胞生长、信号转导、凋亡等生理过程意义重大。飞行时间-二次离子质谱(SIMS)是一种高灵敏的表面质谱成像技术,具有较高的空间分辨率,已被用于细胞及微区域分析。然而,生物有机分子较低的离子化效率限制了ToF-SIMS在单细胞分析中的广泛应用。本研究采用金属/基质增强方法,明显提高了脂质的离子化产率。镀金硅片上磷脂酰胆碱PC(40:0)标样在加入基质后,准分子离子峰强度增大为硅片上的65倍。相应基底上单细胞表面脂质信号同时增强,但由于细胞的不规则形貌以及复杂化学环境影响,脂质信号增强幅度较小。在此基础上,使用延时提取(Delayed extraction,DE)模式克服了细胞形貌带来的影响,进一步增强脂质信号,并获得了高质量的单细胞脂质成像图。此方法为研究细胞代谢及细胞-环境相互作用提供了新的途径。

关键词:   单细胞分析, 脂质, 飞行时间-二次离子质谱, 金属/基质增强, 质谱成像
Key words:   Single cell analysis, Lipids, Time-of-flight secondary ion mass spectrometry, Noble metal/matrix enhancement, Mass spectrometry imaging
[1]

Klepárník K, Foret F. Anal. Chim. Acta, 2013, 800: 12-21. doi: 10.1016/j.aca.2013.09.004

[2]

Costello D A, Villareal V A, Yang P L. ACS Infect. Dis., 2016, 2(11): 852-862. doi: 10.1021/acsinfecdis.6b00086

[3]

Villareal V A, Fu D, Costello D A, Xie X S, Yang P L. ACS Chem. Biol., 2016, 11(7): 1827-1833. doi: 10.1021/acschembio.6b00324

[4]

Angerer T B, Magnusson Y, Landberg G, Fletcher J S. Anal. Chem., 2016, 88(23): 11946-11954. doi: 10.1021/acs.analchem.6b03884

[5]

Dowlatshahi Pour M, Jennische E, Lange S, Ewing A G, Malmberg P. Sci. Rep., 2016, 6: 32797-. doi: 10.1038/srep32797

[6]

Galler K, Brautigam K, Grosse C, Popp J, Neugebauer U. Analyst, 2014, 139(6): 1237-1273. doi: 10.1039/C3AN01939J

[7]

CHENG Xiao-Hong, ZHONG Zhi-Cheng, LI Wang-Nan. Chinese J. Lumin., 2017, (6): 768-779

程晓红, 钟志成, 李望南. 发光学报, 2017, (6): 768-779

[8]

Tekeli I, Aujard I, Trepat X, Jullien L, Raya A, Zalvidea D. Light:Sci. Appl., 2016, 5(6): e16084-. doi: 10.1038/lsa.2016.84

[9]

Shambat G, Kothapalli S R, Provine J, Sarmiento T, Harris J, Gambhir S S, Vučkovic' J. Nano Lett., 2013, 13(11): 4999-5005. doi: 10.1021/nl304602d

[10]

Zhang Q N, Zhong L Y, Tang P, Yuan Y J, Liu S D, Tian J D, Lu X X. Sci. Rep., 2017, 7(1): 2532-

[11]

Feider C L, Elizondo N, Eberlin L S. Anal. Chem., 2016, 88(23): 11533-11541. doi: 10.1021/acs.analchem.6b02798

[12]

Vens-Cappell S, Kouzel I U, Kettling H, Soltwisch J, Bauwens A, Porubsky S, Müthing J, Dreisewerd K. Anal. Chem., 2016, 88(11): 5595-5599. doi: 10.1021/acs.analchem.6b01084

[13]

Stender A S, Marchuk K, Liu C, Sander S, Meyer M W, Smith E A, Neupane B, Wang G, Li J, Cheng J X. Chem. Rev., 2013, 113(4): 2469-2527. doi: 10.1021/cr300336e

[14]

Wagner M. Annu. Rev. Microbiol., 2009, 63: 411-429. doi: 10.1146/annurev.micro.091208.073233

[15]

Norris J L, Caprioli R M. Chem. Rev., 2013, 113(4): 2309-2342. doi: 10.1021/cr3004295

[16]

Bennett R V, Gamage C M, Galhena A S, Fernández F M. Anal. Chem., 2014, 86(8): 3756-3763. doi: 10.1021/ac5007816

[17]

Randall E C, Race A M, Cooper H J, Bunch J. Anal. Chem., 2016, 88(17): 8433-8440. doi: 10.1021/acs.analchem.5b04281

[18]

Bilkey J, Tata A, McKee T D, Porcari A M, Bluemke E, Woolman M, Vwentura M, Eberlin M N, Zarrine-Afsar A, An al. Chem., 2016, 88(24): 12099-12107

[19]

Passarelli M K, Newman C F, Marshall P S, West A, Gilmore I S, Bunch J, Alexander M R, Dollery C T. Anal. Chem., 2015, 87(13): 6696-6702. doi: 10.1021/acs.analchem.5b00842

[20]

Korte A R, Yandeau-Nelson M D, Nikolau B J, Lee Y J. Anal. Bioanal. Chem., 2015, 407(8): 2301-2309. doi: 10.1007/s00216-015-8460-5

[21]

Hua X, Szymanski C, Wang Z, Zhou Y, Ma X, Yu J, Evans J, Orr G, Liu S, Zhu Z, Yu X Y. Integr. Biol., 2016, 8(5): 635-644. doi: 10.1039/C5IB00308C

[22]

Brison J, Robinson M A, Benoit D S W, Muramoto S, Stayton P S, Castner D G. Anal. Chem., 2013, 85(22): 10869-10877. doi: 10.1021/ac402288d

[23]

Passarelli M K, Ewing A G, Winograd N. Anal. Chem., 2013, 85(4): 2231-2238. doi: 10.1021/ac303038j

[24]

Tian H, Sparvero L J, Amoscato A A, Bloom A, Bayir H, Kagan V E, Winograd N. Anal. Chem., 2017, 89(8): 4611-4619. doi: 10.1021/acs.analchem.7b00164

[25]

Haase A, Arlinghaus H F, Tentschert J, Jungnickel H, Graf P, Mantion A, Draude F, Galla S, Plendl J, Goetz M E, Masic A, Meier W, Thünemann A F, Taubert A, Luch A. ACS Nano, 2011, 5(4): 3059-3068. doi: 10.1021/nn200163w

[26]

Cai L S, Xia M C, Wang Z Y, Zhao Y B, Li Z P, Zhang S C, Zhang X R. Anal. Chem., 2017, 89(16): 8372-8376. doi: 10.1021/acs.analchem.7b01629

[27]

Solon E G, Schweitzer A, Stoeckli M, Prideaux B. AAPS J., 2010, 12(1): 11-26. doi: 10.1208/s12248-009-9158-4

[28]

Yokoyama Y, Aoyagi S, Fujii M, Matsuo J, Fletcher J S, Lockyer N P, Vickerman J C, Passarelli M K, Havelund R, Seah M P. Anal. Chem., 2016, 88(7): 3592-3597. doi: 10.1021/acs.analchem.5b04133

[29]

Sheraz Née Rabbani S S, Barber A, Fletcher J S, Lockyer N P, Vickerman J C. Anal. Chem., 2013, 85(12): 5654-5658. doi: 10.1021/ac4013732

[30]

Sheraz Née Rabbani S, Razo I B, Kohn T, Lockyer N P, Vickerman J C. Anal. Chem., 2015, 87(4): 2367-2374. doi: 10.1021/ac504191m

[31]

Vanbellingen Q P, Elie N, Eller M J, Della-Negra S, Touboul D, Brunelle A. Rapid Commun. Mass. Spectrom., 2015, 29(13): 1187-1195. doi: 10.1002/rcm.7210

[32]

Mihara I, Havelund R, Gilmore I S. Anal. Chem., 2017, 89(9): 4781-4785. doi: 10.1021/acs.analchem.7b00511

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目录

金属/基质增强飞行时间-二次离子质谱用于单细胞脂质分析

李好问, 华鑫, 龙亿涛

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