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引用本文:   尹艳艳, 侯丽, 张丽丽, 林学滨, 吴晓苹. 基于双层酶信号放大及纳米功能界面的微囊藻毒素电化学免疫传感器. 分析化学, 2018, 46(4): 493-501. doi:  10.11895/j.issn.0253-3820.171055 [复制]

Citation:   YIN Yan-Yan, HOU Li, ZHANG Li-Li, LIN Xue-Bin, WU Xiao-Ping. Electrochemical Immunosensor for Microcystin-LR Detection Based on Nano-composite Material Immobilization and Enzymatic Amplification. Chinese Journal of Analytical Chemistry, 2018, 46(4): 493-501. doi: 10.11895/j.issn.0253-3820.171055 [复制]

基于双层酶信号放大及纳米功能界面的微囊藻毒素电化学免疫传感器

通讯作者:  吴晓苹, wapple@fzu.edu.cn

收稿日期: 2017-07-01

接受日期: 2017-12-25

出版日期: 2018-04-01

基金项目: 本文系国家自然科学基金项目(No.21375019)和教育部创新团队(二期)项目(No.IRT15R11)资助

Electrochemical Immunosensor for Microcystin-LR Detection Based on Nano-composite Material Immobilization and Enzymatic Amplification

Corresponding author:  WU Xiao-Ping, wapple@fzu.edu.cn

Received Date:  2017-07-01

Accepted Date:  2017-12-25

Published Date:  2018-04-01

Fund Project:  This work was supported by the National Natural Science Foundation of China (No.21375019) and the Ministry of Education Innovation Team Development Plan (Phase Ⅱ) (No.IRT15R11)

设计了一种基于纳米复合膜双层酶信号放大的竞争型电化学免疫传感器,用于检测痕量微囊藻毒素-LR(Microcystin-(leucine-arginine),MCLR)。在多壁碳纳米管修饰玻碳电极上电沉积金纳米粒子形成纳米复合膜,作为MCLR抗体(anti-MCLR)和辣根过氧化物酶(HRP)的固定化载体;引入HRP作为封闭剂或者通过抗体捕获HRP标记的MCLR(HRP-MCLR),起封闭活性位点及协同催化的作用。利用MCLR与HRP-MCLR对纳米复合膜所固载抗体活性位点的竞争结合模式,采用微分脉冲伏安法(DPV)测定电极界面上HRP酶催化过氧化氢(H2O2)氧化对苯二酚(HQ)产生的还原电流,实现MCLR的定量检测。此传感器具有良好的特异性、稳定性与灵敏度,线性检测范围为0.1~100.0 μg/L,检出限为0.038 μg/L(S/N=3),对实际水样的加标回收率为72.9%~117.3%。

关键词:   辣根过氧化物酶, 多壁碳纳米管, 纳米金, 微囊藻毒素, 电化学免疫传感器
Key words:   Horseradish peroxidase, Multi-walled carbon nanotubes, Gold nanoparticles, Microcystin-LR, Electrochemical immunosensor
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基于双层酶信号放大及纳米功能界面的微囊藻毒素电化学免疫传感器

尹艳艳, 侯丽, 张丽丽, 林学滨, 吴晓苹

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