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引用本文:   白敏, 曹晓文, 陈锋, 赵越, 赵永席. 基于量子点-甲酚紫FRET比率探针的活细胞硫化氢成像. 分析化学, 2018, 46(1): 39-47. doi:  10.11895/j.issn.0253-3820.171163 [复制]

Citation:   BAI Min, CAO Xiao-Wen, CHEN Feng, ZHAO Yue, ZHAO Yong-Xi. A Förster Resonance Energy Transfer Ratiometric Probe Based on Quantum Dot-Cresyl Violet for Imaging Hydrogen Sulfide in Living Cells. Chinese Journal of Analytical Chemistry, 2018, 46(1): 39-47. doi: 10.11895/j.issn.0253-3820.171163 [复制]

基于量子点-甲酚紫FRET比率探针的活细胞硫化氢成像

通讯作者:  赵永席, yxzhao@mail.xjtu.edu.cn

收稿日期: 2017-08-10

接受日期: 2017-09-07

出版日期: 2018-01-20

基金项目: 本文系国家自然科学基金(Nos.21475102,31671013)和西安交通大学"青年拔尖人才计划"资助

A Förster Resonance Energy Transfer Ratiometric Probe Based on Quantum Dot-Cresyl Violet for Imaging Hydrogen Sulfide in Living Cells

Corresponding author:  ZHAO Yong-Xi, yxzhao@mail.xjtu.edu.cn

Received Date:  2017-08-10

Accepted Date:  2017-09-07

Published Date:  2018-01-20

Fund Project:  This work was supported by the National Natural Science Foundation of China (Nos. 21475102, 31671013) and the "Young Talent Support Plan" of Xi'an Jiaotong University, China

硫化氢(H2S)作为一种重要的气体信号分子,其细胞水平异常与多种疾病的发生发展密切相关。本研究针对现存荧光探针的胞内非特异性响应问题,发展了一种基于量子点-甲酚紫的荧光能量共振转移(Förster resonance energy transfer,FRET)探针,利用比率荧光分析,实现活细胞内H2S的灵敏成像。通过一步超声乳化法制备量子点纳米球,再结合叠氮甲酚紫(CV-N3)制得无机-有机杂化QDS-N3比率探针。此纳米探针尺寸均一,粒径约为120 nm,酸性稳定性高,且无细胞毒性。H2S可将CV-N3上的N3还原成NH2,生成的氨基甲酚紫(CV-NH2)作为FRET-受体,吸收量子点纳米球的橙色荧光,产生红光信号。通过两个波长的荧光强度的比率分析可有效消除光源强度波动和细胞内复杂环境等的干扰,最终实现了活细胞内H2S成像。

关键词:   量子点, 甲酚紫, 比率荧光, 细胞成像, 硫化氢
Key words:   Quantum dot, Cresyl violet, Ratiometric fluorescence, Cell imaging, Hydrogen sulfide
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基于量子点-甲酚紫FRET比率探针的活细胞硫化氢成像

白敏, 曹晓文, 陈锋, 赵越, 赵永席

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