Citation: ZHANG Yong, LIU Jin-Feng, YI Run-Hao, AI Si-Fan, CHENG Huan-Ren, JIA Wen-Zhi. Synthesis and Application of A Flavone-based Fluorescent Probe for Detection of Hydrazine. Chinese Journal of Analytical Chemistry, 2018, 46(4): 511-516. doi: 10.11895/j.issn.0253-3820.171222 [复制]
Synthesis and Application of A Flavone-based Fluorescent Probe for Detection of Hydrazine
以3-羟基黄酮为荧光团，设计合成了一种用于检测肼的荧光增强型探针ZY8，研究了其光谱性质。结果表明，在Tris-HCl/乙醇溶液（9:1，V/V，pH 7.40）中，ZY8对N2H4具有较好的选择性和专一性。在1.6×10-7~6.2×10-5 mol/L浓度范围内，N2H4的浓度与ZY8的荧光强度呈现良好的线性关系，检出限为1.6×10-7 mol/L。ZY8自身荧光微弱，向其中加入N2H4后，荧光增强9倍左右，而且在波长365 nm的紫外光照射下，溶液的颜色由浅灰绿色变成了明亮的草绿色，可实现对N2H4的可视化识别。它可在接近生理pH范围内对N2H4进行检测，响应快，抗干扰能力强。ZY8制成的试纸能裸眼检测低于mmol/L级别的N2H4溶液，将其应用于不同水样中N2H4的检测，N2H4的回收率在96.0%-104.2%之间，相对标准偏差（RSD）小于4%，表明ZY8在环境污染监测领域有潜在的应用价值。
A fluorescence enhancement probe (ZY8) for the detection of N2H4 was designed and synthesized by employing 3-hydroxyflavone as a fluorophore, and its spectral properties had been investigated. The results showed that ZY8 had relatively good selectivity and specificity to N2H4 in Tris-HCl-ethanol solution (9:1, V/V, pH 7.40). The fluorescent intensity of ZY8 exhibited good linear relationship with concentration of N2H4 in the concentration range of 1.6×10-7 mol/L-6.2×10-5 mol/L, and its detection limit was estimated to be 1.6×10-7 mol/L. ZY8 itself had weak fluorescence, upon addition of N2H4, an approximate 9-fold fluorescence enhancement was observed, and the color of the solution changed from light grayish green to bright grass-green at UV light of 365 nm. So ZY8 might be used to the visual recognition of N2H4. ZY8 could detect N2H4in near-physiological pH range, and it had fast response and strong anti-interference ability. Moreover, ZY8 could be loaded as test paper for naked-eye detection of N2H4 at mmol/L level in water solution, and it was also applied in the determination of N2H4 in various water samples by the standard addition recovery experiments, with the recovery ratio ranged from 96.0% to 104.2%%, and RSD of all < 4%. The results of this study demonstrated that ZY8 had potential application to the detection of N2H4 in the monitoring of environmental pollution.
GB 18061-2000, Hygienic Standard for Hydrazine in Water Sources. National Standards of the People's Republic of China
水源水中肼卫生标准. 中华人民共和国国家标准. GB 18061-2000
GB 3838-2002, Environmental quality standards for surface water. National Standards of the People's Republic of China.
地表水环境质量标准. 中华人民共和国国家标准. GB 3838-2002