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引用本文:   黄华斌, 傅奇, 胡佳, 游其华, 庄峙厦, 杨朝勇, 王小如. 表面电性可控磁珠微流控芯片在DNA提取中的应用. 分析化学, 2018, 46(9): 1372-1378. doi:  10.11895/j.issn.0253-3820.171318 [复制]

Citation:   HUANG Hua-Bin, FU Qi, HU Jia, YOU Qi-Hua, ZHUANG Zhi-Xia, YANG Chao-Yong, WANG Xiao-Ru. Magnetic Microsphere with Surface Charge Switching-based Microfluidic Chip for DNA Extraction. Chinese Journal of Analytical Chemistry, 2018, 46(9): 1372-1378. doi: 10.11895/j.issn.0253-3820.171318 [复制]

表面电性可控磁珠微流控芯片在DNA提取中的应用

通讯作者:  王小如, wxr@hxxy.edu.cn

收稿日期: 2017-10-10

接受日期: 2018-07-08

出版日期: 2018-09-01

基金项目: 本文系国家星火计划项目(No.2015GA721002)、黄土与第四纪地质国家重点实验室开放基金(No.SKLLQG1607)和福建省中青年教师科技计划项目(No.JAT170817)资助

Magnetic Microsphere with Surface Charge Switching-based Microfluidic Chip for DNA Extraction

Corresponding author:  WANG Xiao-Ru, wxr@hxxy.edu.cn

Received Date:  2017-10-10

Accepted Date:  2018-07-08

Published Date:  2018-09-01

Fund Project:  This work was supported by the National Spark Program (No. 2015GA721002), the Open Foundation from State Key Laboratiory of Loess and Quaternary Geology(No. SKLLQG1607)and the Project of Science and Technology for Young Teachers in Fujian Province, China(No. JAT170817)

制备了表面电性可控的氨基化SiO2@Fe3O4磁性复合微球,采用激光刻蚀和热压键合的方法制作了聚甲基丙烯酸甲酯(PMMA)材质的DNA固相萃取芯片,将磁珠灌注于芯片通道中,借助永磁铁固定并控制磁珠,将磁珠芯片应用于人类全血中的基因组DNA提取,优化了提取实验条件,并对提取产物进行凝胶电泳和PCR分析。实验结果表明,磁珠微流控芯片成功地从全血中提取出纯度较高的基因组DNA,提取效率约35%,提取液的凝胶电泳条带与商品化试剂盒提取的基因组DNA一致,提取液可用于进一步的PCR反应。

关键词:   表面电性可控, 磁珠, 微流控芯片, DNA提取
Key words:   Surface charge switching, Magnetic beads, Microfluidic chip, DNA extraction
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表面电性可控磁珠微流控芯片在DNA提取中的应用

黄华斌, 傅奇, 胡佳, 游其华, 庄峙厦, 杨朝勇, 王小如

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