Citation: LU Jin, REN Xue, LIU Xiang-Liang, LIU Ze-Feng. A Metabonomics Study of Cervical Cancer by Ultra Performance Liquid Chromatography Coupled with Quadrupole Orbitrap Tandem Mass Spectrometry. Chinese Journal of Analytical Chemistry, 2018, 46(1): 67-73. doi: 10.11895/j.issn.0253-3820.171412 [复制]
A Metabonomics Study of Cervical Cancer by Ultra Performance Liquid Chromatography Coupled with Quadrupole Orbitrap Tandem Mass Spectrometry
利用超高效液相色谱-四极杆-静电场轨道阱质谱联用（UHPLC-Q-Orbitrap MS）方法对宫颈癌（Cervical cancer，CC）患者和健康人（Healthy control，HC）的尿液进行分析，研究宫颈癌患者尿液中的潜在标志物，为其发病机制和诊断提供科学依据。筛选11例宫颈癌患者（Age（45.7±5.6）years）及11例健康人（Age（45.9±3.2）years）尿液样本，采用液相色谱-质谱联用技术对尿液进行测定，通过主成分分析（PCA）和偏最小二乘法-判别分析（OPLS-DA）处理数据，结果表明，两组人群代谢轮廓有显著差别，发现并鉴定了12种潜在的生物标志物，提示特定的肿瘤代谢途径中潜在的代谢标志物可能在宫颈癌发生发展中发挥重要作用。
This work aims to analyze and compare the potential biomarkers between cervical cancer (CC) patients and healthy controls (HC). The urine samples of 11 CC patients (age (45.65 ±5.6) years) and 11 HC patients (age (45.9 ±3.2) years) were collected. The metabolites of urine samples from CC and HC were analyzed by using ultra performance liquid chromatography coupled with quadrupole orbitrap tandem mass spectrometry (UHPLC-Q-Orbitrap MS/MS), which could provide evidence for early diagnose and disease pathway. The LC-MS data of urines were analyzed by principal components analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) to identify the potential biomarkers. Urine samples of CC patients were successfully discirminated from those of healthy controls. A total of twelve significant metabolites were found and identified as potential biomarkers according to the established UHPLC-Q-Orbitrap MS and MS/MS method. Identification of biomarkers between CC and HC patients may play an important role in the study of mechanism of UC and its pathway.