基于反相液相色谱-串联质谱的鹿茸模拟胃肠道消化的肽谱及生物活性研究

引用本文: 于洋, 晏嘉泽, 靳艳. 基于反相液相色谱-串联质谱的鹿茸模拟胃肠道消化的肽谱及生物活性研究. 分析化学, 2018, 46(4): 601-608. doi: 10.11895/j.issn.0253-3820.171421 [复制]

Citation: YU Yang, YAN Jia-Ze, JIN Yan. Identification of Peptide Profiling of Deer Antler by Reversed Phase Liquid Chromatography-Tandem Mass Spectrometry and Bioactivities in Simulated Gastrointestinal Digestion. Chinese Journal of Analytical Chemistry, 2018, 46(4): 601-608. doi: 10.11895/j.issn.0253-3820.171421 [复制]

基于反相液相色谱-串联质谱的鹿茸模拟胃肠道消化的肽谱及生物活性研究

于洋, 晏嘉泽, 靳艳

中国科学院大连化学物理研究所, 中国科学院分离分析化学重点实验室, 大连 116023

通讯作者: 靳艳, yanjin@dicp.ac.cn

收稿日期: 2017-11-13

接受日期: 2018-01-25

出版日期: 2018-04-01

基金项目: 本文系中国科学院战略生物资源服务网络计划项目（No.ZSTH-029）和吉林省院合作项目（No.2016SYHZ0020）资助

Identification of Peptide Profiling of Deer Antler by Reversed Phase Liquid Chromatography-Tandem Mass Spectrometry and Bioactivities in Simulated Gastrointestinal Digestion

YU Yang, YAN Jia-Ze, JIN Yan

Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical and Physics, Chinese Academy of Sciences, Dalian 116023, China

Corresponding author: JIN Yan, yanjin@dicp.ac.cn

Received Date: 2017-11-13

Accepted Date: 2018-01-25

Published Date: 2018-04-01

利用反相液相色谱-串联质谱（Reversed phase liquid chromatography-tandem mass spectrometry，RPLC-MS/MS）对鹿茸蛋白经过模拟消化的肽谱进行分析，同时考察了消化产物的体外活性。从鹿茸水提物（Antler aqueous extract，AAE）、鹿茸水提物消化产物（Aqueous extract digest，AED）和鹿茸的粉末消化产物（Powder digest，PD）中分别鉴定到23、417和389条肽段，其中源于胶原蛋白的肽分别有15、146和75条。以血管紧张素转换酶（Angiotensin converting enzyme，ACE）、二肽基肽酶Ⅳ（Dipeptidyl peptidase Ⅳ，DPP-Ⅳ）、脯氨酰内切酶（Prolyl endopeptidase，PEP）抑制活性和抗氧化活性为指标考察AAE、AED和PD的体外生物活性，发现其活性强弱关系为AAE < AED < PD。结果表明，经过模拟消化后，AED释放出更丰富的胶原蛋白肽，而PD具有更好的生物活性，说明食用鹿茸粉末和鹿茸水提物互为补充，各具优势。

关键词: 鹿茸, 模拟胃肠道消化, 反相液相色谱-串联质谱, 肽谱, 生物活性

Reversed phase liquid chromatography-tandem mass spectrometry (RPLC-MS/MS) was utilized to investigate peptide profiling and bioactivities of antler aqueous extract (AAE), digested antler aqueous extract (AED) and powder (PD). A total of 23, 417 and 389 peptides, as well as 15, 146 and 75 collagen peptides were identified from AAE, AED and PD, respectively. Angiotensin converting enzyme (ACE) inhibitory activity, dipeptidyl peptidase Ⅳ (DPP-Ⅳ) inhibitory activity, prolyl endopeptidase (PEP) inhibitory activity and antioxidant activity were used to evaluate the bioactivities of AAE, AED and PD, and it was found that the sequence of their bioactivities was AAE < AED < PD. All the results above proved that AED released more collagen peptides and PD possessed better biological activities. It suggests that the two edible ways of antler are complemented each other and have their own advantages.

Key words: Deer antler, Simulated gastrointestinal digestion, Reversed phase liquid chromatography-tandem mass spectrometry, Peptide profiling, Biological activities

[1]	JI Jing-Xian, QIAN Jing, HUANG Feng-Jie, WU Wu-Tong, GAO Xiang-Dong. Chinese J. Biochem. Pharm., 2009, 30(2): 141-143 吉静娴, 钱璟, 黄凤杰, 吴梧桐, 高向东. 中国生化药物杂志, 2009, 30(2): 141-143
[2]	Jeon B T, Kim S J, Lee S M, Park P J, Sung S H, Kim J M, Moon S H. Mammalian Biology-Zeitschrift für Säugetierkunde, 2009, 74(5): 374-380. doi: 10.1016/j.mambio.2008.07.005
[3]	Park H J, Lee D H, Park S G, Lee S C, Cho S Y, Kim H K, Kim J J, Bae H S, Park B C. Proteomics, 2004, 4(11): 3642-3653. doi: 10.1002/(ISSN)1615-9861
[4]	Gao L, Tao D Y, Shan Y C, Liang Z, Zhang L H, Huo Y S, Zhang Y K. J. Chromatogr. B, 2010, 878(32): 3370-3374. doi: 10.1016/j.jchromb.2010.10.022
[5]	Sui Z G, Yuan H M, Liang Z, Zhao Q, Wu Q, Xia S M, Zhang L H, Huo Y S, Zhang Y K. Talanta, 2013, 107: 189-194. doi: 10.1016/j.talanta.2013.01.015
[6]	Lee S H, Yang H W, Ding Y L, Wang Y M, Jeon Y J, Moon S H, Jeon B T, Sung S H. Excli J., 2015, 14: 1122-1132
[7]	Wu F F, Li H Q, Jin L J, Li X Y, Ma Y S, You J S, Li S Y, Xu Y P. J. Ethnopharmacol., 2013, 145(2): 403-415. doi: 10.1016/j.jep.2012.12.008
[8]	Zhao L, Luo Y C, Wang C T, Ji B P. Nat. Prod. Commun., 2011, 6(11): 1683-1688
[9]	Lee M R, Yun B S, Zhang D L, Liu L, Wang Z, Wang C L, Gu L J, Wang C Y, Mo E K, Ly S Y, Sung C K. Food Sci. Biotechnol., 2010, 19(3): 655-661. doi: 10.1007/s10068-010-0092-0
[10]	Xin J L, Zhang Y, Zhang L Z, Lin Y, Zhou Q L. Chem. Res. Chinese U., 2013, 29(5): 924-928. doi: 10.1007/s40242-013-3070-2
[11]	Zha E H, Li X X, Li D D, Guo X S, Gao S Y, Yue X Q. Int. Immunopharmacol., 2013, 16(2): 210-213. doi: 10.1016/j.intimp.2013.02.027
[12]	Zhang Z Y, Liu X F, Duan L X, Li X, Zhang Y, Zhou Q L. Acta Biochim. Pol., 2011, 58(3): 297-302
[13]	Sánchez-Rivera L, Diezhandino I, Gómez-Ruiz J Á, Fresno J M, Miralles B, Recio I. Electrophoresis, 2014, 35(11): 1627-1636. doi: 10.1002/elps.v35.11
[14]	Jin Y, Yu Y, Qi Y X, Wang F J, Yan J Z, Zou H F. J. Proteomics, 2016, 141: 24-46. doi: 10.1016/j.jprot.2016.04.010
[15]	Picariello G, Ferranti P, Fierro O, Mamone G, Caira S, Luccia A D, Monica S, Addeo F. J. Chromatogr. B, 2010, 878(3-4): 295-308. doi: 10.1016/j.jchromb.2009.11.033
[16]	Yu Y, Jin Y, Wang F J, Yan J Z, Qi Y X, Ye M L. Food Res. Int., 2017, 96: 182-190. doi: 10.1016/j.foodres.2017.04.002
[17]	GUO Jing, YAN Jia-Ze, GUO Ming, JIN Yan. Chinese Journal of Chromatography, 2014, 32(3): 284-289 郭静, 晏嘉泽, 郭明, 靳艳. 色谱, 2014, 32(3): 284-289
[18]	Zhu J, Sun Z, Cheng K, Chen R, Ye M L, Xu B, Sun D G, Wang L M, Liu J, Wang F J, Zou H F. J. Proteome Res., 2014, 13(3): 1713-1721. doi: 10.1021/pr401200h
[19]	Murray B A, Walsh D J, FitzGerald R J. J. Biochem. Bioph. Meth., 2004, 59(2): 127-137. doi: 10.1016/j.jbbm.2003.12.009
[20]	Hernndez-Ledesma B, Hsieh C C. Bioactive Food Peptides In Health And Disease, 2013, : 207-208
[21]	Cahlíková L, Hulová L, Hrabinová M, Chlebek J, Hoštálková A, Adamcová M, Šafratová M, Jun D, Opletal L, Ločárek M, Macáková K. Fitoterapia, 2015, 103: 192-196. doi: 10.1016/j.fitote.2015.04.004
[22]	Awika J M, Rooney L W, WU Xian-Li, Prior R L, Cisneros-Zevallos L. J. Agric. Food Chem., 2003, 51(23): 6657-6662. doi: 10.1021/jf034790i
[23]	WANG Xuan, ZHAO Lei, WANG Cheng-Tao. Food Sci. Industry, 2014, 35(12): 81-86 王璇, 赵磊, 王成涛. 食品工业科技, 2014, 35(12): 81-86
[24]	Jin Y, Yan J Z, Yu Y, Qi Y X. J. Funct. Foods, 2015, 18(1): 344-357
[25]	Foltz M, Meynen E E, Bianco V, Platerink C V, Koning T M M G, Kloek J. J. Nutr., 2007, 137(4): 953-958. doi: 10.1093/jn/137.4.953
[26]	Nongonierma A B, FitzGerald R J. Food Chem., 2014, 145: 845-852. doi: 10.1016/j.foodchem.2013.08.097
[27]	Nongonierma A B, FitzGerald R J. Peptides, 2013, 39: 157-163. doi: 10.1016/j.peptides.2012.11.016
[28]	Fülöp V, Böcskei Z, Polgár L. Cell, 1998, 94(2): 161-170. doi: 10.1016/S0092-8674(00)81416-6
[29]	Garcia-Horsman J A, Mannisto P T, Venalainen J I. Neuropeptides, 2007, 41(1): 1-24. doi: 10.1016/j.npep.2006.10.004
[30]	Farvin K H S, Baron C P, Nielsen N S, Jacobsen C. Food Chem., 2010, 123(4): 1081-1089. doi: 10.1016/j.foodchem.2010.05.067
[31]	Farvin K H S, Baron C P, Nielsen N S, Otte J, Jacobsen C. Food Chem., 2010, 123(4): 1090-1097. doi: 10.1016/j.foodchem.2010.05.029
[32]	Basirico L, Catalani E, Morera P, Cattaneo S, Stuknyte M, Bernabucci U, de Noni I, Nardone A. J. Dairy Sci., 2015, 98(11): 7595-7601. doi: 10.3168/jds.2015-9801

研究报告

基于反相液相色谱-串联质谱的鹿茸模拟胃肠道消化的肽谱及生物活性研究

于洋, 晏嘉泽, 靳艳

关键词: 鹿茸, 模拟胃肠道消化, 反相液相色谱-串联质谱, 肽谱, 生物活性

Identification of Peptide Profiling of Deer Antler by Reversed Phase Liquid Chromatography-Tandem Mass Spectrometry and Bioactivities in Simulated Gastrointestinal Digestion

YU Yang, YAN Jia-Ze, JIN Yan

Key words: Deer antler, Simulated gastrointestinal digestion, Reversed phase liquid chromatography-tandem mass spectrometry, Peptide profiling, Biological activities

计量

PDF下载量(5)
文章访问量(211)
HTML全文浏览量(5)

作者相关

在本站搜索
于洋晏嘉泽靳艳
在百度学术搜索
于洋晏嘉泽靳艳
在万方数据库搜索
于洋晏嘉泽靳艳
在CNKI搜索
于洋晏嘉泽靳艳