引用本文: 于洋, 晏嘉泽, 靳艳. 基于反相液相色谱-串联质谱的鹿茸模拟胃肠道消化的肽谱及生物活性研究. 分析化学, 2018, 46(4): 601-608. doi: 10.11895/j.issn.0253-3820.171421 [复制]
Citation: YU Yang, YAN Jia-Ze, JIN Yan. Identification of Peptide Profiling of Deer Antler by Reversed Phase Liquid Chromatography-Tandem Mass Spectrometry and Bioactivities in Simulated Gastrointestinal Digestion. Chinese Journal of Analytical Chemistry, 2018, 46(4): 601-608. doi: 10.11895/j.issn.0253-3820.171421 [复制]
基于反相液相色谱-串联质谱的鹿茸模拟胃肠道消化的肽谱及生物活性研究
Identification of Peptide Profiling of Deer Antler by Reversed Phase Liquid Chromatography-Tandem Mass Spectrometry and Bioactivities in Simulated Gastrointestinal Digestion
利用反相液相色谱-串联质谱(Reversed phase liquid chromatography-tandem mass spectrometry,RPLC-MS/MS)对鹿茸蛋白经过模拟消化的肽谱进行分析,同时考察了消化产物的体外活性。从鹿茸水提物(Antler aqueous extract,AAE)、鹿茸水提物消化产物(Aqueous extract digest,AED)和鹿茸的粉末消化产物(Powder digest,PD)中分别鉴定到23、417和389条肽段,其中源于胶原蛋白的肽分别有15、146和75条。以血管紧张素转换酶(Angiotensin converting enzyme,ACE)、二肽基肽酶Ⅳ(Dipeptidyl peptidase Ⅳ,DPP-Ⅳ)、脯氨酰内切酶(Prolyl endopeptidase,PEP)抑制活性和抗氧化活性为指标考察AAE、AED和PD的体外生物活性,发现其活性强弱关系为AAE < AED < PD。结果表明,经过模拟消化后,AED释放出更丰富的胶原蛋白肽,而PD具有更好的生物活性,说明食用鹿茸粉末和鹿茸水提物互为补充,各具优势。
Reversed phase liquid chromatography-tandem mass spectrometry (RPLC-MS/MS) was utilized to investigate peptide profiling and bioactivities of antler aqueous extract (AAE), digested antler aqueous extract (AED) and powder (PD). A total of 23, 417 and 389 peptides, as well as 15, 146 and 75 collagen peptides were identified from AAE, AED and PD, respectively. Angiotensin converting enzyme (ACE) inhibitory activity, dipeptidyl peptidase Ⅳ (DPP-Ⅳ) inhibitory activity, prolyl endopeptidase (PEP) inhibitory activity and antioxidant activity were used to evaluate the bioactivities of AAE, AED and PD, and it was found that the sequence of their bioactivities was AAE < AED < PD. All the results above proved that AED released more collagen peptides and PD possessed better biological activities. It suggests that the two edible ways of antler are complemented each other and have their own advantages.
基于反相液相色谱-串联质谱的鹿茸模拟胃肠道消化的肽谱及生物活性研究
Identification of Peptide Profiling of Deer Antler by Reversed Phase Liquid Chromatography-Tandem Mass Spectrometry and Bioactivities in Simulated Gastrointestinal Digestion
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