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引用本文:   于洋, 晏嘉泽, 靳艳. 基于反相液相色谱-串联质谱的鹿茸模拟胃肠道消化的肽谱及生物活性研究. 分析化学, 2018, 46(4): 601-608. doi:  10.11895/j.issn.0253-3820.171421 [复制]

Citation:   YU Yang, YAN Jia-Ze, JIN Yan. Identification of Peptide Profiling of Deer Antler by Reversed Phase Liquid Chromatography-Tandem Mass Spectrometry and Bioactivities in Simulated Gastrointestinal Digestion. Chinese Journal of Analytical Chemistry, 2018, 46(4): 601-608. doi: 10.11895/j.issn.0253-3820.171421 [复制]

基于反相液相色谱-串联质谱的鹿茸模拟胃肠道消化的肽谱及生物活性研究

通讯作者:  靳艳, yanjin@dicp.ac.cn

收稿日期: 2017-11-13

接受日期: 2018-01-25

出版日期: 2018-04-01

基金项目: 本文系中国科学院战略生物资源服务网络计划项目(No.ZSTH-029)和吉林省院合作项目(No.2016SYHZ0020)资助

Identification of Peptide Profiling of Deer Antler by Reversed Phase Liquid Chromatography-Tandem Mass Spectrometry and Bioactivities in Simulated Gastrointestinal Digestion

Corresponding author:  JIN Yan, yanjin@dicp.ac.cn

Received Date:  2017-11-13

Accepted Date:  2018-01-25

Published Date:  2018-04-01

利用反相液相色谱-串联质谱(Reversed phase liquid chromatography-tandem mass spectrometry,RPLC-MS/MS)对鹿茸蛋白经过模拟消化的肽谱进行分析,同时考察了消化产物的体外活性。从鹿茸水提物(Antler aqueous extract,AAE)、鹿茸水提物消化产物(Aqueous extract digest,AED)和鹿茸的粉末消化产物(Powder digest,PD)中分别鉴定到23、417和389条肽段,其中源于胶原蛋白的肽分别有15、146和75条。以血管紧张素转换酶(Angiotensin converting enzyme,ACE)、二肽基肽酶Ⅳ(Dipeptidyl peptidase Ⅳ,DPP-Ⅳ)、脯氨酰内切酶(Prolyl endopeptidase,PEP)抑制活性和抗氧化活性为指标考察AAE、AED和PD的体外生物活性,发现其活性强弱关系为AAE < AED < PD。结果表明,经过模拟消化后,AED释放出更丰富的胶原蛋白肽,而PD具有更好的生物活性,说明食用鹿茸粉末和鹿茸水提物互为补充,各具优势。

关键词:   鹿茸, 模拟胃肠道消化, 反相液相色谱-串联质谱, 肽谱, 生物活性
Key words:   Deer antler, Simulated gastrointestinal digestion, Reversed phase liquid chromatography-tandem mass spectrometry, Peptide profiling, Biological activities
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基于反相液相色谱-串联质谱的鹿茸模拟胃肠道消化的肽谱及生物活性研究

于洋, 晏嘉泽, 靳艳

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