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引用本文:   柳检, 罗立强. 芹菜根细胞的超微结构与铅形态特征分析. 分析化学, 2018, 46(9): 1479-1485. doi:  10.11895/j.issn.0253-3820.181253 [复制]

Citation:   LIU Jian, LUO Li-Qiang. Analysis of Ultrastructure of Root Cell and Lead Speciation in Celery. Chinese Journal of Analytical Chemistry, 2018, 46(9): 1479-1485. doi: 10.11895/j.issn.0253-3820.181253 [复制]

芹菜根细胞的超微结构与铅形态特征分析

通讯作者:  罗立强, luoliqiang@cags.ac.cn

收稿日期: 2018-04-16

接受日期: 2018-06-27

出版日期: 2018-09-01

基金项目: 本文系国家重点研发计划项目(No.2016YFC0600603)、国家自然科学基金项目(Nos.20775018,41201527)、国家高新技术研究发展计划(863)项目(No.2007AA06Z124)和中国地质调查项目(No.DD20160340)资助

Analysis of Ultrastructure of Root Cell and Lead Speciation in Celery

Corresponding author:  LUO Li-Qiang, luoliqiang@cags.ac.cn

Received Date:  2018-04-16

Accepted Date:  2018-06-27

Published Date:  2018-09-01

Fund Project:  This work was supported by the National Key Research and Development Program of China (No. 2016YFC0600603), the National Natural Science Foundation of China (Nos. 20775018, 41201527), the National High Technology Research and Development Program of China (No. 2007AA06Z124) and the Pproject of China Geological Survey (No. DD20160340)

植物细胞是植物响应重金属胁迫的重要生化反应场所,探索细胞层级中铅(Pb)的分布与形态转化是揭示有害元素对蔬菜毒性作用机理的重要途径。本研究以芹菜为例,利用能量色散透射电子显微镜(TEM-EDS)和X射线吸收近边结构谱(XANES)技术,考察了Pb胁迫后芹菜根细胞的超微结构、元素分布和形态转化。研究发现,Pb胁迫显著改变了芹菜根细胞的超微结构,导致胞间间隙加大、细胞壁增厚和质膜变糙;芹菜根、茎和叶对Pb具有显著且不同的形态转化功能,可将无机铅(Pb(NO32)转化为有机铅形态;芹菜根细胞中62.7%的Pb以Pb5(PO43Cl形态沉积在根细胞壁,根和茎中有机铅(Pb(Ac)2·3H2O)约占34.4%-37.3%,叶中总有机铅(Pb(C17H35COO)2和Pb(Ac)2·3H2O)占63.3%。结果表明,芹菜细胞的Pb-磷酸盐来源于植物体的生物合成,而非外界根际环境,芹菜根际环境的Pb能够以Pb-Ac的络合物形式进入根细胞,一部分转化成Pb5(PO43Cl沉积于细胞壁,实现Pb的解毒和耐受,少部分经茎长程运输至叶片以Pb-大分子有机酸/磷酸盐的形式贮存。

关键词:   蔬菜, 细胞, 超微结构, 铅形态, X-射线吸收近边结构谱
Key words:   Vegetable, Cell, Ultrastructure, Lead speciation, X-ray absorption near edge spectroscopy
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芹菜根细胞的超微结构与铅形态特征分析

柳检, 罗立强

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