会员登陆: 用户名:  密码: 验证码:
首页 杂志概况 投稿须知 在线投稿 在线阅读 征订启事 广告服务 行业资讯 企业动态 资料中心  专访报道 会展信息 ENGLISH

引用本文:   孙艳红, 魏佳, 王振新, 孟宪瑛. 富G寡聚核苷酸修饰的金纳米粒子与细胞相互作用的研究. 分析化学, 2018, 46(9): 1357-1362. doi:  10.11895/j.issn.0253-3820.181293 [复制]

Citation:   SUN Yan-Hong, WEI Jia, WANG Zhen-Xin, MENG Xian-Ying. Study on Interaction of G-rich Oligonucleotides Modified Gold Nanoparticles with Cells. Chinese Journal of Analytical Chemistry, 2018, 46(9): 1357-1362. doi: 10.11895/j.issn.0253-3820.181293 [复制]

富G寡聚核苷酸修饰的金纳米粒子与细胞相互作用的研究

通讯作者:  孟宪瑛, xy6823@163.com

收稿日期: 2018-05-04

接受日期: 2018-06-27

出版日期: 2018-09-01

基金项目: 本文系国家自然科学基金项目(Nos.81571737,21475126)资助

Study on Interaction of G-rich Oligonucleotides Modified Gold Nanoparticles with Cells

Corresponding author:  MENG Xian-Ying, xy6823@163.com

Received Date:  2018-05-04

Accepted Date:  2018-06-27

Published Date:  2018-09-01

Fund Project:  This work was supported by the National Natural Science Foundation of China (Nos. 81571737, 21475126)

通过Au-S键将两种链长一致、序列不同的富鸟嘌呤(G)单链寡聚核苷酸PolyG1和PolyG2分别修饰到13 nm金纳米粒子(GNPs)表面,合成了两种单链寡聚核苷酸(ssDNAs)与GNPs复合物(PolyG1-GNPs和PolyG2-GNPs)。所合成的PolyG1-GNPs和PolyG2-GNPs在复杂分散介质中具有良好的胶体稳定性。采用紫外-可见吸收光谱、细胞透射电镜和电感耦合等离子体质谱等分析方法,考察ssDNA序列对PolyG1-GNPs和PolyG2-GNPs与细胞相互作用的影响。结果表明,PolyG1-GNPs和PolyG2-GNPs均具有较低的细胞毒性,并表现与能量相关的细胞内吞行为,ssDNA的序列决定PolyG1-GNPs和PolyG2-GNPs的细胞吞噬量及其在细胞内的分散状态,具有G4二级结构的PolyG2能够显著增加细胞对其所修饰的GNPs的吞噬量和GNPs在细胞中的稳定性。

关键词:   富G单链寡聚核苷酸, 活细胞, 金纳米粒子, 相互作用
Key words:   G-rich single-stranded oligonucleotides, Living cells, Gold nanoparticles, Interaction
[1]

Mirkin C A, Letsinger R L, Mucic R C, Storhoff J J. Nature, 1996, 38: 607-609

[2]

Elghanian R, Storhoff J J, Mucic R C, Letsinger R L, Mirkin C A. Science, 1997, 277(5329): 1078-1081. doi: 10.1126/science.277.5329.1078

[3]

Rosi N L, Giljohann D A, Thaxton C S, Lytton-Jean A K R, Han M S, Mirkin C A. Science, 2006, 312(5776): 1027-1030. doi: 10.1126/science.1125559

[4]

Rosi N L, Mirkin C A. Chem. Rev., 2005, 105: 1547-1562. doi: 10.1021/cr030067f

[5]

Zhao W, Ali M M, Brook M A, Li Y F. Angew. Chem. Int. Edit., 2008, 47: 6330-6337. doi: 10.1002/anie.v47:34

[6]

Wang Z X, Ma L. Coord. Chem. Rev., 2009, 253: 1607-1618. doi: 10.1016/j.ccr.2009.01.005

[7]

Chen N, Li J, Song H Y, Chao J, Huang Q, Fan C H. Acc. Chem. Res., 2014, 47(6): 1720-1730. doi: 10.1021/ar400324n

[8]

Koo K M, Sina A A, Carrascosa L G, Shiddiky M J A, Trau M. Anal. Methods, 2015, 7: 7042-7054. doi: 10.1039/C5AY01479D

[9]

Qin L, Zeng G, Lai C, Huang D, Xu P, Zhang C, Cheng M, Liu X, Liu S, Li B, Yi H. Coord. Chem. Rev., 2018, 359: 1-31. doi: 10.1016/j.ccr.2018.01.006

[10]

LI Zhu-Heng, MA Li-Na, LIU Dian-Jun, WANG Zhen-Xin. Chinese J. Appl. Chem., 2016, 33(9): 1009-1016

李铸衡, 马立娜, 刘殿骏, 王振新. 应用化学, 2016, 33(9): 1009-1016

[11]

Charbgoo F, Nejabat M, Abnous K, Soltani F, Taghdisi S M, Alibolandi M, Shier W T, Steele T W J, Ramezani M. J. Controlled Release, 2018, 272: 39-53. doi: 10.1016/j.jconrel.2018.01.002

[12]

Halo T L, McMahon K M, Angeloni N L, Xu Y, Wang W, Chinen A B, Malin D, Strekalova E, Cryns V L, Cheng C, Mirkin C A, Thaxton C S. Proc. Natl. Acad. Sci. USA, 2014, 111(48): 17104-17109. doi: 10.1073/pnas.1418637111

[13]

Randeria P S, Jones M R, Kohlstedt K L, Banga R J, de la Cruz M O, Schatz G C, Mirkin C A. J. Am. Chem. Soc., 2015, 137(10): 3486-3489. doi: 10.1021/jacs.5b00670

[14]

Zhao X J, Hilliard L R, Mechery S J, Wang Y P, Bagwe R P, Jin S G, Tan W H. Proc. Natl. Acad. Sci. USA, 2004, 101(42): 15027-15032. doi: 10.1073/pnas.0404806101

[15]

Choi C H J, Hao L, Narayan S P, Auyeung E, Mirkin C A. Proc. Natl. Acad. Sci. USA, 2013, 110(19): 7625-7630. doi: 10.1073/pnas.1305804110

[16]

Narayan S P, Choi C H J, Hao L, Calabrese C M, Auyeung E, Zhang C, Goor O J G M, Mirkin C A. Small, 2015, 11(33): 4173-4182. doi: 10.1002/smll.v11.33

[17]

Rosi N L, Mirkin C A. Chem. Rev., 2005, 105(4): 1547-1562. doi: 10.1021/cr030067f

[18]

Li J, Huang J, Yang X, Yang Y, Quan K, Xie N, Wu Y, Ma C, Wang K. Talanta, 2018, 183: 11-17. doi: 10.1016/j.talanta.2018.02.036

[19]

Paramasivan S, Rujan I, Bolton P H. Methods, 2007, 43(4): 324-331. doi: 10.1016/j.ymeth.2007.02.009

[20]

Kuznetsov N V. Methods Mol. Biol., 2013, 986: 41-56. doi: 10.1007/978-1-62703-311-4

[21]

Kumar S, Aaron J, Sokolov K. Nat. Protoc., 2008, 3(2): 314-320. doi: 10.1038/nprot.2008.1

[22]

Zheng D, Giljohann D A, Chen D L, Massich M D, Wang X Q, Iordanov H, Mirkin C A, Paller A S. Proc. Natl. Acad. Sci. USA, 2012, 109(30): 11975-11980. doi: 10.1073/pnas.1118425109

计量
  • PDF下载量(18)
  • 文章访问量(134)
  • HTML全文浏览量(11)

目录

富G寡聚核苷酸修饰的金纳米粒子与细胞相互作用的研究

孙艳红, 魏佳, 王振新, 孟宪瑛

Figures and Tables