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引用本文:   贾永梅, 娄筱叮, 夏帆. 核酸荧光探针在单细胞成像中的应用研究. 分析化学, 2018, 46(9): 1329-1338. doi:  10.11895/j.issn.0253-3820.181340 [复制]

Citation:   JIA Yong-Mei, LOU Xiao-Ding, XIA Fan. Fluorescent Bioprobe Based on Nucleic Acid for Live Cell Imaging. Chinese Journal of Analytical Chemistry, 2018, 46(9): 1329-1338. doi: 10.11895/j.issn.0253-3820.181340 [复制]

核酸荧光探针在单细胞成像中的应用研究

通讯作者:  夏帆, xiafan@hust.edu.cn

收稿日期: 2018-05-21

接受日期: 2018-07-11

出版日期: 2018-09-01

基金项目: 本文系国家自然科学基金项目(No.21705071)、广东省自然科学基金项目(No.2016A030310362)和岭南师范学院自然科学引进人才项目(No.ZL1611)资助

Fluorescent Bioprobe Based on Nucleic Acid for Live Cell Imaging

Corresponding author:  XIA Fan, xiafan@hust.edu.cn

Received Date:  2018-05-21

Accepted Date:  2018-07-11

Published Date:  2018-09-01

Fund Project:  This work was supported by the National Natural Science Foundation of China (No. 201705071), the Natural Science Foundation of Gugangdong Province, China (No. 2016A030310362), and the Initiatory Financial Support from Lingnan Normal University (No. ZL1611)

单细胞成像研究技术具有观测定位精准、独特的时间和空间分辨率高等特点,成为分子水平上准确、实时、原位监测细胞内生物活性物质信号变化及观测细胞形态的重要手段。在进行单细胞成像研究时,细胞内大多数生物活性物质自身无法产生易被仪器或肉眼所捕获的信号,通常需要使用生物探针进入细胞内特异性识别生物活性物质。生物探针与特定的生物活性物质结合,形成稳定的复合物,产生相应信号变化。通过监测生物探针信号变化,实现对细胞内生物活性物质准确、实时、原位监测。生物探针的响应信号有多种,其中荧光信号不仅具有直观、操作简单、选择性好、灵敏度高、无需参比、不受电磁场的影响、可远程实时在线自动监测等特性,还具备对细胞进行静态观察、对细胞内分子动力学进行动态监测、对亚细胞结构进行定位和对蛋白质分子的相互作用进行研究等优点,在细胞成像技术研究中具有重要地位。本文综述了近五年来核酸荧光探针在单细胞成像中的应用研究进展,讨论了存在的问题,对发展方向进行了展望。

关键词:   核酸荧光探针, 细胞成像, 生物传感, 分子检测, 评述
Key words:   Fuorescent bioprobe, Cell imaging, Biosensor, Molecular detection, Review
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核酸荧光探针在单细胞成像中的应用研究

贾永梅, 娄筱叮, 夏帆

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