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引用本文:   张君才, 陈佑宁, 卫引茂. 高容量硼酸亲和磁性纳米粒子的制备及其在邻羟基生物分子富集中的应用. 分析化学, 2018, 46(9): 1464-1471. doi:  10.11895/j.issn.0253-3820.181369 [复制]

Citation:   ZHANG Jun-Cai, CHENG You-Ning, WEI Yin-Mao. Preparation of Boron Affinity Magnetic Adsorbent with High Adsorption Capacity and Its Application in Extraction of Cis-Diol Biomolecules. Chinese Journal of Analytical Chemistry, 2018, 46(9): 1464-1471. doi: 10.11895/j.issn.0253-3820.181369 [复制]

高容量硼酸亲和磁性纳米粒子的制备及其在邻羟基生物分子富集中的应用

通讯作者:  张君才, xyjuncai@163.com

收稿日期: 2018-06-02

接受日期: 2018-07-19

出版日期: 2018-09-01

基金项目: 本文系国家自然科学基金项目(Nos.21475104,21775121)和陕西省现代分离科学重点实验室基金项目(No.17JS134)资助

Preparation of Boron Affinity Magnetic Adsorbent with High Adsorption Capacity and Its Application in Extraction of Cis-Diol Biomolecules

Corresponding author:  ZHANG Jun-Cai, xyjuncai@163.com

Received Date:  2018-06-02

Accepted Date:  2018-07-19

Published Date:  2018-09-01

Fund Project:  This work was supported by the National Natural Science Foundation of China (Nos. 21475104, 21775121) and the Key Laboratory of Modern Separation Science in Shaanxi Province, China (No. 17JS134)

硼亲和吸附法是分离富集邻羟基物质的重要方法。为提高吸附剂的吸附容量,本研究采用聚乙烯亚胺修饰磁性纳米粒子,增加粒子表面引发剂的密度,再通过表面引发原子转移自由基聚合法(SI-ATRP)将3-丙烯酰基苯硼酸原位聚合在纳米粒子表面,制备了一种高密度聚合物分子刷型的硼酸亲和磁性纳米粒子吸附剂。采用磁性分散固相萃取法对非邻羟基和邻羟基物质的混合液进行富集,发现此吸附剂对邻羟基小分子和生物大分子具有良好的吸附特异性;采用吸附等温线法测定了吸附剂的吸附容量,发现此吸附剂对邻苯二酚、腺苷和卵清蛋白的吸附容量分别为(151±32)μmol/g、(123±18)μmol/g和1.5 μmol/g,远高于传统吸附剂的吸附容量。采用所制备的硼酸亲和磁性纳米粒子对尿液中4种核苷和蛋清中的糖蛋白进行萃取,结果表明,此吸附剂能够有效去除生物样品中的干扰物,且对核苷具有较高的萃取回收率(83.8%-108.7%,RSD < 15%),对糖蛋白有特异性富集作用,说明此吸附剂在生物样品的选择性富集中具有良好应用潜能。

关键词:   磁性分离, 硼亲和吸附剂, 核苷, 糖蛋白, 生物分析
Key words:   Magnetic separation, Boron affinity adsorbent, Nucleoside, Glycoprotein, Biological analysis
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高容量硼酸亲和磁性纳米粒子的制备及其在邻羟基生物分子富集中的应用

张君才, 陈佑宁, 卫引茂

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