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引用本文:   孙伯禄, 蔡金颖, 李玳, 苟晓丹, 苟于强, 李文, 胡芳弟. 用于毛蕊异黄酮痕量分析的电化学传感器研究. 分析化学, 2019, 47(2): 271-280. doi:  10.19756/j.issn.0253-3820.181574 [复制]

Citation:   SUN Bo-Lu , CAI Jin-Ying , LI Dai , GOU Xiao-Dan , GOU Yu-Qiang , LI Wen , HU Fang-Di . Fabrication of Electrochemical Sensor Modified with Porous Graphene for Determination of Trace Calycosin. Chinese Journal of Analytical Chemistry, 2019, 47(2): 271-280. doi: 10.19756/j.issn.0253-3820.181574 [复制]

用于毛蕊异黄酮痕量分析的电化学传感器研究

收稿日期: 2018-09-05

基金项目: 本文系甘肃省科技厅科技重大专项项目(No.17ZD2FA009)、兰州市科技局专利转化项目(No.2017-4-119)、兰州市成果转化基地项目(No.2016-2-80)、国家重点研发计划项目(No.2018YFC1706300)和国家中药标准化项目(No.ZYBZH-Y-GS-10)资助

Fabrication of Electrochemical Sensor Modified with Porous Graphene for Determination of Trace Calycosin

Received Date:  2018-09-05

Fund Project:  This work was supported by the Major Science and Technology Project of Gansu Provincial Science and Technology Department (No. 17ZD2FA009), the Patent Conversion Project of Lanzhou Science and Technology Bureau (No. 2017-4-119), the Achievement Transformation Base Project of Lanzhou (No. 2016-2-80), the National Key Research and Development Program of China (No. 2018YFC1706300) and the National Traditional Chinese Medicine Standardization Project (No. ZYBZH-Y-GS-10).

通过简单的热解过程成功制备了结构优化的多孔石墨烯(PG),并用于构建复杂体系中毛蕊异黄酮(CYS)痕量分析的电化学传感器。采用拉曼光谱法、傅里叶红外光谱分析法及扫描电子显微镜对制备过程进行了表征。循环伏安法(CV)和交流阻抗法(EIS)研究了PG修饰的玻碳电极(PG@GCE)的电化学行为,结果表明,PG有效增加了修饰电极的有效比表面积和电极表面的电子传递速率,中药电活性成分CYS在PG@GCE表面具有良好的电化学响应。在优化的条件下,采用差分脉冲伏安法(DPV)对CYS进行分析,CYS在1.8×10-7-4.4×10-5 mol/L浓度范围内具有良好的线性关系,检出限为5.8×10-8 mol/L(S/N=3)。将此传感器用于中药黄芪、红芪的提取物及血浆样品中CYS的分析时,呈现良好的分析性能。本研究为中药黄芪和红芪中指标性成分的快速分析及生物样品中CYS的痕量分析提供了一种新颖的分析手段,也进一步深化了PG在药物分析领域的应用。

关键词:   多孔石墨烯, 毛蕊异黄酮, 电化学传感器, 痕量分析
Key words:   Porous graphene, Calycosin, Electrochemical sensor, Trace analysis
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用于毛蕊异黄酮痕量分析的电化学传感器研究

孙伯禄, 蔡金颖, 李玳, 苟晓丹, 苟于强, 李文, 胡芳弟

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