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引用本文:   方涵书, 郎明非, 孙晶. 细胞周期分析新方法. 分析化学, 2019, 47(9): 1293-1301. doi:  10.19756/j.issn.0253-3820.191225 [复制]

Citation:   FANG Han-Shu , LANG Ming-Fei , SUN Jing . New Methods for Cell Cycle Analysis. Chinese Journal of Analytical Chemistry, 2019, 47(9): 1293-1301. doi: 10.19756/j.issn.0253-3820.191225 [复制]

细胞周期分析新方法

收稿日期: 2019-04-23

基金项目: 本文系国家自然科学基金项目(No.21505013)和大连市科技创新基金项目(No.2018J13SN087)资助

New Methods for Cell Cycle Analysis

Received Date:  2019-04-23

Fund Project:  This work was supported by the National Natural Science Foundation of China (No. 21505013) and the Dalian Science and Technology Innovation Funds (No. 2018J13SN087).

细胞周期是细胞生命活动的基本过程,对细胞周期的分析能更深入地了解疾病发生的本质。目前,分析细胞周期的方法以流式细胞术为主,主要是通过检测细胞内DNA含量和细胞周期特异性蛋白而分析细胞周期。随着分析技术的发展,细胞周期分析在图像分析、单细胞分析、减少样本量、提高通量等方面不断地提高和改进,近年出现了许多分析细胞周期的新方法,如成像流式细胞术、质谱流式细胞术、微流控技术、拉曼光谱法等,这些方法极大地丰富了细胞周期的检测方法,提高了细胞周期测量的精确度。本文对细胞周期的新分析方法进行了介绍,并对其发展方向进行了展望。

关键词:   细胞周期, 细胞周期蛋白, DNA含量, 微流控技术, 流式细胞术, 评述
Key words:   Cell cycle, Cell cycle proteins, DNA contents, Microfluidics, Flow cytometry, Review
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细胞周期分析新方法

方涵书, 郎明非, 孙晶

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