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引用本文:   车峥, 岳诗雨, 吴南, 徐嘉蔚, 陈晴. 碲钨酸盐磁性纳米复合材料选择性分离鸡蛋清中卵清蛋白. 分析化学, 2019, 47(9): 1302-1308. doi:  10.19756/j.issn.0253-3820.191249 [复制]

Citation:   CHE Zheng , YUE Shi-Yu , WU Nan , XU Jia-Wei , CHEN Qing . Preparation of Tungstotellurate (Ⅵ)-coated Magnetic Nanoparticles for Separation and Purification of Ovalbumin in Egg White. Chinese Journal of Analytical Chemistry, 2019, 47(9): 1302-1308. doi: 10.19756/j.issn.0253-3820.191249 [复制]

碲钨酸盐磁性纳米复合材料选择性分离鸡蛋清中卵清蛋白

收稿日期: 2019-05-02

基金项目: 本文系国家自然基金项目(No.21804093)、辽宁省自然科学基金项目(No.20180550052)、沈阳医学院博士科研启动基金项目(No.20186068)、沈阳医学院大学生课题(No.20189042)和辽宁省过敏性疾病过敏原致病机制研究重点实验室项目(No.[2018]30)资助

Preparation of Tungstotellurate (Ⅵ)-coated Magnetic Nanoparticles for Separation and Purification of Ovalbumin in Egg White

Received Date:  2019-05-02

Fund Project:  This work was supported by the National Natural Science Foundation of China (No.21804093), the Natural Science Foundation of Liaoning Province, China (No.20180550052), the Science and Technology Foundation for the Shenyang Medical College (No.20186068), the Undergraduate Scientific Research Project for the Shenyang Medical College (No. 20189042), and the Funds of the Key Laboratory of Research on Pathogenesis of Allergen Provoked Allergic Disease of Liaoning Province (No.[2018]30).

将碲钨酸盐(Na6[TeW6O24]·22H20,TeW)通过静电作用负载到支状聚乙烯亚胺(PEI)修饰的磁性纳米粒子(MNP)表面,得到新型的基于多金属氧酸盐的复合材料(TeW@MNP),采用红外光谱、能谱、扫描电镜等对其进行表征。蛋白质吸附研究结果表明,该磁性纳米复合材料对卵清蛋白具有选择性吸附作用。考察了吸附时间、离子强度、洗脱剂种类和用量等对分离纯化效率的影响,在pH 4.0时,0.5 mg TeW@MNP对200 μL 100 μg/mL卵清蛋白的吸附效率为91.6%,最大理论吸附容量为373.4 mg/g,以0.1% SDS为洗脱剂时,对卵清蛋白的洗脱效率为98.1%。SDS-PAGE实验表明,采用此复合材料可成功地从卵清样品中分离出纯度较高的卵清蛋白。本研究以TeW@MNP复合物为固相萃取剂,建立了从鸡蛋清中分离纯化卵清蛋白的方法,拓宽了多金属氧酸盐的应用范围。

关键词:   碲钨酸盐, 磁性纳米材料, 卵清蛋白, 固相萃取
Key words:   Tungstotellurate, Magnetic nanoparticles, Ovalbumin, Solid-phase extraction
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目录

碲钨酸盐磁性纳米复合材料选择性分离鸡蛋清中卵清蛋白

车峥, 岳诗雨, 吴南, 徐嘉蔚, 陈晴

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