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引用本文:   石维平, 蔡杰, 杨雅妮, 罗欢欢, 刘冰倩, 付秋平. 便携式血糖仪检测汞离子. 分析化学, 2019, 47(9): 1337-1343. doi:  10.19756/j.issn.0253-3820.191311 [复制]

Citation:   SHI Wei-Ping , CAI Jie , YANG Ya-Ni , LUO Huan-Huan , LIU Bing-Qian , FU Qiu-Ping . Portable Glucose Meter for Detection of Mercury(Ⅱ) Ion. Chinese Journal of Analytical Chemistry, 2019, 47(9): 1337-1343. doi: 10.19756/j.issn.0253-3820.191311 [复制]

便携式血糖仪检测汞离子

收稿日期: 2019-05-09

基金项目: 本文系国家自然科学基金项目(Nos.21605029,21864007)、贵州省科技计划项目(黔科合平台人才[2017]5788)、贵州省科技计划项目(黔科合基础(2016)1032)、贵州大学引进人才科研项目(贵大人基合字(2015)40)和贵阳市财政支持贵阳学院学科与硕士点建设项目[HC-2019]资助

Portable Glucose Meter for Detection of Mercury(Ⅱ) Ion

Received Date:  2019-05-09

Fund Project:  This work was supported by the National Natural Science Foundation of China (Nos. 21605029, 21864007), the Science and Technology Foundation of Guizhou Province ([2017]5788 Qian Ke He Platform for talents), the Science and Technology Foundation of Guizhou Province ([2016]1032 Qian Ke He Ji Chu), the Introduction of Talents Scientific Research Foundation of Guizhou University ([2015]40 Gui Da Ren Ji He Zi), the Discipline and Master's Site Construction Project of Guiyang University by Guiyang City Financial Support Guiyang University[HC-2019].

利用T-Hg2+-T错配对汞离子(Hg2+)的特异性识别,以Fe3O4纳米磁珠(Fe3O4 nano-magnetic beads,MB)为反应平台,便携式血糖仪(Portable blood glucose meter,PGM)为检测手段,构建了一种简便灵敏的Hg2+检测方法。采用柠檬酸钠还原法制备金纳米颗粒(Gold nanoparticle,AuNPs),其表面用末端巯基化且富含胸腺嘧啶(T)的寡核苷酸探针(S1)和葡萄糖氧化酶(Glucose oxidase,GOx)修饰。在Hg2+存在下,功能化AuNPs通过T-Hg2+-T配对被捕获到富T探针(S2)修饰的MB上,AuNPs表面的GOx将葡萄糖(Glucose,Glu)氧化成葡萄糖酸和H2O2,使反应液中Glu含量降低,由PGM定量检测Glu含量。优化得到最佳实验条件:S2与GOx体积比为1:3,Hg2+反应时间为2 h,Glu水解时间为1 h。在最优条件下,Hg2+浓度在0.05~8.00 nmol/L范围内,体系PGM响应与的Hg2+浓度对数呈良好的线性关系,检出限为0.02 nmol/L (3σ)。实际水样中Hg2+的加标回收率为97.8%-113.7%,相对标准偏差为0.2%-1.3%,可以满足实际水样中Hg2+的检测要求。

关键词:   T-Hg2+-T, 四氧化三铁纳米磁珠, 金纳米颗粒, 便携式血糖仪, 汞离子(Ⅱ)
Key words:   Thymine-Hg2+-Thymine, Fe3O4 nano-magnetic beads, Gold nanoparticles, Portable glucose meter, Mercury ion(Ⅱ)
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便携式血糖仪检测汞离子

石维平, 蔡杰, 杨雅妮, 罗欢欢, 刘冰倩, 付秋平

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