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引用本文:   蓝皓慧, 王昕岑, 张晓梅, 张鸿伟, 丛培旭, 徐杰, 薛长湖. 超高效液相色谱-串联四极杆飞行时间高分辨质谱用于太平洋鳕鱼脂质轮廓分析. 分析化学, 2020, 48(7): 903-911. doi:  10.19756/j.issn.0253-3820.191595 [复制]

Citation:   LAN Hao-Hui , WANG Xin-Cen , ZHANG Xiao-Mei , ZHANG Hong-Wei , CONG Pei-Xu , XU Jie , XUE Chang-Hu . Lipid Profile Analysis Method Based on Ultra High Performance Liquid Chromatography-Triple Time-of-Flight Tandem Mass Spectrometry and Its Application in Gadus Macrocephalus. Chinese Journal of Analytical Chemistry, 2020, 48(7): 903-911. doi: 10.19756/j.issn.0253-3820.191595 [复制]

超高效液相色谱-串联四极杆飞行时间高分辨质谱用于太平洋鳕鱼脂质轮廓分析

通讯作者:  徐杰, xuejie9@ouc.edu.cn

收稿日期: 2019-10-11

基金项目: 本文系国家重点研发计划专项(No.2018YFD0901101)资助

Lipid Profile Analysis Method Based on Ultra High Performance Liquid Chromatography-Triple Time-of-Flight Tandem Mass Spectrometry and Its Application in Gadus Macrocephalus

Corresponding author:  XU Jie , xuejie9@ouc.edu.cn

Received Date:  2019-10-11

Fund Project:  This work was supported by the National Key R&D Program of China (No. 2018YFD0901101).

建立了超高效液相色谱-串联四极杆飞行时间高分辨质谱(UPLC-Triple TOF-MS/MS)分析脂质轮廓的方法。色谱柱选用ACQUITY UPLC CSH C18柱(150 mm×2.1 mm,1.7 μm),流动相A为乙腈-水(65:35,V/V),流动相B为异丙醇-乙腈(85:15,V/V),两者均含5 mmol/L甲酸铵和0.1%甲酸;质谱采用IDA自动二级扫描模式。13类脂质标准品在一定浓度范围内具有良好的线性关系,线性相关系数(R2)均大于0.995,检出限(LOD)为0.001~0.25 μmol/L,定量限(LOQ)为0.0025~0.2 μmol/L,相对标准偏差(RSD)均小于10%,大部分标准品的回收率大于80%。采用氯仿-甲醇(2:1,V/V)提取太平洋鳕鱼肌肉组织中的脂质,共鉴定出498个脂质分子种类,包括255个磷脂、52个鞘脂、191个甘油酯,主要亚类分别为磷脂酰乙醇胺、鞘磷脂、甘油二酯。本方法具有高灵敏、高分辨和高通量等优点,可用于太平洋鳕鱼脂质轮廓的分析,为脂质组学在水产品领域中的应用奠定了方法学基础。

关键词:   脂质轮廓, 超高效液相色谱-串联四极杆飞行时间高分辨质谱, 磷脂, 鞘脂, 太平洋鳕鱼
Key words:   Lipid profile, Ultra high performance liquid chromatography-triple time-of-flight tandem mass spectrometry, Phospholipids, Sphingolipids, Gadus macrocephalus
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目录

超高效液相色谱-串联四极杆飞行时间高分辨质谱用于太平洋鳕鱼脂质轮廓分析

蓝皓慧, 王昕岑, 张晓梅, 张鸿伟, 丛培旭, 徐杰, 薛长湖

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