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引用本文:   赵宏, 王玉迎, 赵良娟, 宓捷波, 赵化冰, 张峰, 冯峰, 曾静, 王洪彬, 董志珍. 金黄色葡萄球菌候选特征肽的靶向分析. 分析化学, 2020, 48(7): 921-927. doi:  10.19756/j.issn.0253-3820.191703 [复制]

Citation:   ZHAO Hong , WANG Yu-Ying , ZHAO Liang-Juan , MI Jie-Bo , ZHAO Hua-Bing , ZHANG Feng , FENG Feng , ZENG Jing , WANG Hong-Bin , DONG Zhi-Zhen . Targeted Analysis of Candidate Characteristic Peptides of Staphylococcus Aureus. Chinese Journal of Analytical Chemistry, 2020, 48(7): 921-927. doi: 10.19756/j.issn.0253-3820.191703 [复制]

金黄色葡萄球菌候选特征肽的靶向分析

通讯作者:  董志珍, dongzhizhen@customs.gov.cn

收稿日期: 2019-11-29

基金项目: 本文系国家重点研发计划项目(No.2017YFC1601600)资助

Targeted Analysis of Candidate Characteristic Peptides of Staphylococcus Aureus

Corresponding author:  DONG Zhi-Zhen , dongzhizhen@customs.gov.cn

Received Date:  2019-11-29

Fund Project:  This work was supported by the National Key Research and Development Project Program of China (No.2017YFC1601600).

采用液相色谱-串联质谱联用的靶向分析技术,通过检测食源性致病菌金黄色葡萄球菌水解生成的特征肽,实现食品中致病菌的高效检测。针对文献报道的金黄色葡萄球菌的9种候选特征肽,验证了这些特征肽的靶向分析方法,考察和比较了分析效能。结果表明,基于多反应监测模式的靶向分析方法灵敏度高,更适于特征肽的检测;确定了每个特征肽最佳的碎片离子质量,进一步的生物样品分析结果表明,I1(AYLAVPAAPK)、I2(DYNSPTLIGWVK)、I5(AGYTVNNTPK)和I6(SISSGYTSGR)更适合作为金黄色葡萄球菌的候选特征肽。本研究为大规模验证候选特征肽的鉴定效果奠定了基础,为特征肽检测策略在食源性致病菌检测中的应用提供了参考。

关键词:   食源性致病菌, 金黄色葡萄球菌, 特征肽, 液相色谱-质谱联用
Key words:   Food-borne pathogenic bacteria, Staphylococcus aureus, Characteristic peptides, Liquid chromatography-tandem mass spectrometry
[1]

Fridkin S K, Hageman J C, Morrison M, Sanza L T, Farley M M. N. Engl. J. Med., 2005,352(14):1436-1444

[2]

Jarraud S. Infect. Immun., 2002,70(2):631-641

[3]

Zmantar T, Kouidhi B, Miladi H, Bakhrouf A. BMC Res. Notes, 2011, 4(1):453

[4]

Gilligan K, Shipley M, Stiles B, Hadfield T L, Ibrahim M S. Mol. Cell. Probes, 2000,14(2):71-78

[5]

Tan T Y, Corden S, Barnes R, Cookson B. J. Clin. Microbiol., 2001,39(12):4529-4531

[6]

Wei C, Zhong J, Hu T, Zhao X.3 Biotech, 2018,8(1):76

[7]

Ward A C, Hannah A J, Kendrick S L, Tucker N P, MacGregor G, Connolly P. Biosens. Bioelectron., 2018,110:65-70

[8]

Robin P. Clin. Infect. Dis., 2013,57(4):564-572

[9]

Dubois D, Leyssene D, Chacornac J P, Kostrzewa M, Schmit P O, Talon R, Bonnet R, Delmas J. J. Clin. Microbiol., 2010,48(3):941-945

[10]

Zhu H B, Hu Y F, Yao S Y, Shen C S, Yue P X. J. Food Safety, 2015,6(5):1583-1590

[11]

Kruh-Garcia N A, Wolfe L M, Chaisson L H, Worodria W O, Nahid P, Schorey J S, Davis J L, Dobos K M. PLoS One, 2014,9(7):e103811

[12]

Jamnongkan W, Lebrilla C B, Barboza M, Techasen A, Loilome W, Sithithaworn P, Khuntikeo N, Pairojkul C, Chamadol N, Thanan R, Yongvanit P. Biomolecules, 2019,9(10):538

[13]

Rees J C, Pierce C L, Schieltz D M, Barr J R. Anal. Chem., 2015,87(13):6769-6777

[14]

Charretier Y, Dauwalder O, Franceschi C, Degoutcharmette E, Zambardi G, Cecchini T, Bardet C, Lacoux X, Dufour P, Veron L. Sci. Rep., 2015,5:13944

[15]

Boyle-Vavra S, Challapalli M, Daum R S. Antimicrob. Agents Chemother., 2003,47(6):2036-2039

[16]

Kuroda M, Ohta T, Uchiyama I, Baba T, Yuzawa H, Kobayashi I, Cui L Z, Oguchi A, Aoki K, Nagai Y, Lian J Q, Ito T, Kanamori M, Matsumaru H, Maruyama A, Murakami H, Hosoyama A, Mizutani-Ui Y, Takahashi N K, Sawano T, Inoue R, Kaito C, Sekimizu K, Hirakawa H, Kuhara S, Goto S, Yabuzaki J, Kanehisa M, Yamashita A, Oshima K, Furuya K, Yoshino C, Shiba T, Hattori M, Ogasawara N, Hayashi H, Hiramatsu K. Lancet, 2001,357(9264):1225-1240

[17]

Pokkunuri I, Champney W S. RNA Biol., 2007,4(3):147-153

[18]

Holden M T G, Feil E J, Lindsay J A, Peacock S J, Day N P J, Enright M C, Foster T J, Moore C E, Hurst L, Atkin R, Barron A, Bason N, Bentley S D, Chillingworth C, Chillingworth T, Churcher C, Clark L, Corton C, Cronin A, Doggett J, Dowd L, Feltwell T, Hance Z, Harris B, Hauser H, Holroyd S, Jagels K, James K D, Lennard N, Line A, Mayes R, Moule S, Mungall K, Ormond D, Quail M A, Rabbinowitsch E, Rutherford K, Sanders M, Sharp S, Simmonds M, Stevens K, Whitehead S, Barrell B G, Spratt B G, Parkhill J. Proc. Natl. Acac Sci. USA, 2004,101(26):9786-9791

[19]

McCallum N, Spehar G, Bischoff M, Berger-Bachi B. BBA-Gen. Subjects, 2006,1760(10):1475-1481

[20]

Waanders L F, Almeida R, Prosser S, Cox J, Eikel D, Allen M H, Schultz G A, Mann M. Mol. Cell. Proteomics, 2008,7(8):1452-1459

[21]

Godzien J, Ciborowski M, Martínez-Alcázar M P, Samczuk P, Kretowski A, Barbas C. J. Proteome Res., 2015,14(8):3204-3216

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金黄色葡萄球菌候选特征肽的靶向分析

赵宏, 王玉迎, 赵良娟, 宓捷波, 赵化冰, 张峰, 冯峰, 曾静, 王洪彬, 董志珍

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