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引用本文:   田润, 陶晴, 卞晓军, 朱福琳, 颜娟. 基于杂交链式反应的适配体传感器用于卡那霉素的比色检测. 分析化学, 2020, 48(5): 608-614. doi:  10.19756/j.issn.0253-3820.191708 [复制]

Citation:   TIAN Run , TAO Qing , BIAN Xiao-Jun , ZHU Fu-Lin , YAN Juan . Aptasensor Based on Hybrid Chain Reaction for Colorimetric Detection of Kanamycin. Chinese Journal of Analytical Chemistry, 2020, 48(5): 608-614. doi: 10.19756/j.issn.0253-3820.191708 [复制]

基于杂交链式反应的适配体传感器用于卡那霉素的比色检测

收稿日期: 2019-12-02

基金项目: 本文系国家自然科学基金项目(No.21775102)资助

Aptasensor Based on Hybrid Chain Reaction for Colorimetric Detection of Kanamycin

Received Date:  2019-12-02

Fund Project:  This work was supported by the National Natural Science Foundation of China (No. 21775102).

基于醛基化磁珠颗粒(Aldehyde magnetic beads,AMBs)和DNA杂交链式反应(Hybridization chain reaction,HCR)的信号放大策略,设计了检测卡那霉素(Kanamycin,Kana)的比色适配体生物传感器(Aptasensor)。制备了Mbs@DNA复合物,其上的卡那霉素适配体链与卡那霉素发生特异识别后,释放出互补链,互补链含有设计好的引发序列,可作为信号探针,引发HCR反应,生成的双链DNA(Double-stranded DNA,dsDNA)表现出强负电性,与溶液中带负电金纳米颗粒(Goldnanoparticles,AuNPs)相互排斥,在高盐条件下,AuNPs的稳定性被破坏而发生聚集,导致溶液的颜色从红色变为紫色,吸收光谱也发生变化。本方法检测卡那霉素的线性范围为1.6~32.0 nmol/L,检出限为0.9 nmol/L(S/N=3);实际样品牛奶和蜂蜜样品中卡那霉素的加标回收率在96.0%~105.0%之间,相对标准偏差在3.3%~5.0%之间,说明本方法实用性良好。本方法对卡那霉素的检测具有较高的灵敏度和特异性,操作简单便捷,适用于现场检测等。同时,根据不同待测物具有与其特异性结合的适配体,本方法为其它目标物的检测提供了参考,在食品安全检测与监管中具有良好的应用前景。

关键词:   卡那霉素, 杂交链式反应, 核酸适配体, 金纳米颗粒, 食品安全
Key words:   Kanamycin, Hybridization chain reaction, Aptamer, Gold nanoparticles, Food safety
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基于杂交链式反应的适配体传感器用于卡那霉素的比色检测

田润, 陶晴, 卞晓军, 朱福琳, 颜娟

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