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引用本文:   杨妍, 尹盈爱, 董益阳. 纳米金-适配体比色传感法检测玉米油中黄曲霉毒素B1. 分析化学, 2020, 48(5): 676-684. doi:  10.19756/j.issn.0253-3820.191728 [复制]

Citation:   YANG Yan , YIN Ying-Ai , DONG Yi-Yang . Detection of Aflatoxin B1 in Corn Oil by Colorimetric Gold Nanoparticles-Aptasensor. Chinese Journal of Analytical Chemistry, 2020, 48(5): 676-684. doi: 10.19756/j.issn.0253-3820.191728 [复制]

纳米金-适配体比色传感法检测玉米油中黄曲霉毒素B1

收稿日期: 2019-12-10

基金项目: 本文系"十三五"国家重点研发计划NQI专项项目(No.2016YFF0203703)资助

Detection of Aflatoxin B1 in Corn Oil by Colorimetric Gold Nanoparticles-Aptasensor

Received Date:  2019-12-10

Fund Project:  This work was supported by the National Key Research and Development Program of China (No.2016YFF0203703).

黄曲霉毒素B1(Aflatoxin B1,AFB1)是真菌或霉菌产生的有毒代谢物,通过污染农作物和食品从而对人体健康造成严重威胁。本研究建立了纳米金(AuNPs)-适配体比色传感法,采用不同序列长度的AFB1适配体检测玉米油中AFB1。通过优化适配体浓度、NaCl浓度和体系反应温度,进一步提高体系检测灵敏度。在最佳条件下,50(B50)和80(A80)个碱基长度的适配体建立的AuNPs-适配体比色传感法的检出限分别为13.55和10.56 ng/mL,线性范围均为20~1000 ng/mL,且选择性良好。基于B50和A80适配体比色传感法检测玉米油中AFB1的加标回收率分别为102.4%~104.9%和98.1%~109.1%。结果表明,基于B50和A80建立的AuNPs比色传感法均可用于玉米油中AFB1特异性鉴定。本研究为现场快速检测和高效筛查食品中AFB1污染提供了技术支持,为开发针对其它靶标的适配体传感检测方法奠定了基础。

关键词:   黄曲霉毒素B1, 适配体, 纳米金-适配体比色传感法, 玉米油
Key words:   Aflatoxin B1, Aptamer, Colorimetric gold nanoparticles-aptasensor, Corn oil
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纳米金-适配体比色传感法检测玉米油中黄曲霉毒素B1

杨妍, 尹盈爱, 董益阳

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