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引用本文:   姜浩, 吕雪飞, 赵可心. 基于微流控芯片的核酸适配体筛选技术研究进展. 分析化学, 2020, 48(5): 590-600,684. doi:  10.19756/j.issn.0253-3820.191775 [复制]

Citation:   JIANG Hao , LYU Xue-Fei , ZHAO Ke-Xin . Progress of Aptamer Screening Techniques Based on Microfluidic Chips. Chinese Journal of Analytical Chemistry, 2020, 48(5): 590-600,684. doi: 10.19756/j.issn.0253-3820.191775 [复制]

基于微流控芯片的核酸适配体筛选技术研究进展

收稿日期: 2019-12-31

Progress of Aptamer Screening Techniques Based on Microfluidic Chips

Received Date:  2019-12-31

适配体(Aptamer)是通过指数富集的配体系统进化技术(SELEX)筛选得到的,可与靶标分子以高亲和力特异性结合的单链DNA或RNA,在生物分离分析、临床诊断和疾病靶向治疗等领域应用广泛。适配体的发展与筛选技术的进步密切相关,以SELEX为基础,研究者开发了磁珠SELEX和毛细管电泳SELEX等多种适配体体外筛选技术,但这些方法存在筛选轮次多、筛选周期长和样品消耗量大、对小分子筛选效率低等缺点。微流控芯片具有体积小、高通量和易集成等特点,基于微流控芯片的SELEX技术在一定程度上可解决上述问题,实现适配体快速、高通量的体外筛选。本文在总结SELEX及其关键技术要点的基础上,重点评述了基于微流控和微阵列芯片SELEX技术的研究进展,并对SELEX技术中未来的发展方向进行了总结和展望。

关键词:   适配体, 筛选, 指数富集的配体系统进化技术, 微流控芯片, 微阵列芯片, 评述
Key words:   Aptamer, Screening, Systematic evolution of ligands by exponential enrichment, Microfluidic chip, Microarray chip, Review
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基于微流控芯片的核酸适配体筛选技术研究进展

姜浩, 吕雪飞, 赵可心

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