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引用本文:   何浩延, 黄恩奇, 黎柱均, 舒博文, 徐邦牢, 刘大渔. 微流控芯片液滴数字化分析用于快速定量检测细菌. 分析化学, 2020, 48(7): 855-862. doi:  10.19756/j.issn.0253-3820.201022 [复制]

Citation:   HE Hao-Yan , HUANG En-Qi , LI Zhu-Jun , SHU Bo-Wen , XU Bang-Lao , LIU Da-Yu . Droplet Digital Microfluidic Assay for Rapid Bacterium Quantitation. Chinese Journal of Analytical Chemistry, 2020, 48(7): 855-862. doi: 10.19756/j.issn.0253-3820.201022 [复制]

微流控芯片液滴数字化分析用于快速定量检测细菌

通讯作者:  徐邦牢, banglaoxu@163.com

收稿日期: 2020-01-09

基金项目: 本文系国家自然科学基金项目(Nos.81871726,21804044)和广州市科创委科学研究专项(Nos.201707010277,201904010237,201904010413)资助

Droplet Digital Microfluidic Assay for Rapid Bacterium Quantitation

Corresponding author:  XU Bang-Lao , banglaoxu@163.com

Received Date:  2020-01-09

Fund Project:  This work was supported by the National Natural Science Foundation of China (Nos. 81871726, 21804044).

现有细菌定量检测多依赖专业实验室,检测周期较长。针对此问题,本研究基于微流控芯片液滴数字化分析,建立了一种细菌定量检测方法。采用具有平行液滴分析单元的微流控芯片,其特点在于使用了注射器真空驱动液滴产生方法。借助刃天青显色反应引起的荧光强度改变,可指示液滴内活性细菌的存在。通过计算细菌阳性液滴的比例,采用泊松分布算法,计算出原始样品中的细菌密度。实验结果表明,本方法可在3.5 h内完成细菌定量分析,动态检测范围为105~108 CFU/mL,相对标准偏差(RSD)低于5%。本方法具有操作简便和分析速度快的优势,有望广泛用于细菌快速定量检测。

关键词:   微流控芯片, 液滴数字化分析, 注射器真空, 细菌, 定量检测
Key words:   Microfluidic chip, Droplet digital assay, Syringe vacuum, Bacterium, Quantitation detection
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微流控芯片液滴数字化分析用于快速定量检测细菌

何浩延, 黄恩奇, 黎柱均, 舒博文, 徐邦牢, 刘大渔

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