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引用本文:   赵颖, 王楠, 高华龙, 郭子轩, 陆安祥, 郭晓军, 卢静华, 栾云霞. 核酸适配体亲和柱净化-高效液相色谱法测定莲子中黄曲霉毒素B1. 分析化学, 2020, 48(5): 662-669. doi:  10.19756/j.issn.0253-3820.201030 [复制]

Citation:   ZHAO Ying , WANG Nan , GAO Hua-Long , GUO Zi-Xuan , LU An-Xiang , GUO Xiao-Jun , LU Jing-Hua , LUAN Yun-Xia . Determination of Aflatoxin B1 in Lotus Seed by High Performance Liquid Chromatography with Aptamer Affinity Column for Purification and Enrichment. Chinese Journal of Analytical Chemistry, 2020, 48(5): 662-669. doi: 10.19756/j.issn.0253-3820.201030 [复制]

核酸适配体亲和柱净化-高效液相色谱法测定莲子中黄曲霉毒素B1

收稿日期: 2020-01-11

基金项目: 本文系北京市农林科学院科技创新专项建设基金项目(No.KJCX20170401)和青年基金项目(No.QNJJ201903)资助

Determination of Aflatoxin B1 in Lotus Seed by High Performance Liquid Chromatography with Aptamer Affinity Column for Purification and Enrichment

Received Date:  2020-01-11

Fund Project:  This work was supported by the Science and Technology Innovation Special Construction Funded Program of Beijing Academy of Agriculture and Forestry Sciences (No.KJCX20170401) and the Beijing Agricultural Forestry Academy Youth Foundation (No. QNJJ201903).

以特异性的核酸适配体作为识别元件,制备了黄曲霉毒素B1 (AFB1)的适配体亲和柱(AAC),用于AFB1的特异性识别和富集。对AAC的载样液中有机溶剂含量、载样液的体积和淋洗体积等条件进行了优化。此AAC吸附AFB1的柱容量达到(334.6±18.2) ng,且AAC可重复使用20次。莲子样品经AAC净化富集后,用高效液相色谱-光化学衍生荧光法检测,外标法定量,AFB1检出限达到0.05 ng/mL,回收率为91.8%~108.6%。本方法为食品、农产品和中药等复杂基质中痕量毒素的提取富集和分析提供了新的参考,具有良好的应用前景。

关键词:   适配体亲和柱, 黄曲霉毒素B1, 固相萃取, N-羟基硫代琥珀酰亚胺活化的琼脂糖, 莲子
Key words:   Aptamer affinity column, Aflatoxin B1, Solid-phase extraction, N-Hydroxy succinimide-activated sepharose, Lotus seed
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目录

核酸适配体亲和柱净化-高效液相色谱法测定莲子中黄曲霉毒素B1

赵颖, 王楠, 高华龙, 郭子轩, 陆安祥, 郭晓军, 卢静华, 栾云霞

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