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引用本文:   袁敏, 钱世权, 曹慧, 徐斐, 叶泰, 于劲松, 郭文, 吴嘉颖, 阿提坎·吾斯曼. 一种超灵敏检测镉离子的核酸适配体电化学传感器. 分析化学, 2020, 48(12): 1701-1708. doi:  10.19756/j.issn.0253-3820.201072 [复制]

Citation:   YUAN Min , QIAN Shi-Quan , CAO Hui , XU Fei , YE Tai , YU Jin-Song , GUO Wen , WU Jia-Ying , A Ti-kan·Wu-siman . An Ultra-sensitive Electrochemical Aptasensor for Detection of Cd2+. Chinese Journal of Analytical Chemistry, 2020, 48(12): 1701-1708. doi: 10.19756/j.issn.0253-3820.201072 [复制]

一种超灵敏检测镉离子的核酸适配体电化学传感器

收稿日期: 2020-02-13

基金项目: 本文系国家自然科学基金项目(Nos.31671934,61501295)、科技部"十三五"计划项目(No.2017YFC1600603)和上海市科委重点攻关项目(Nos.18391901200,17391901500)资助

An Ultra-sensitive Electrochemical Aptasensor for Detection of Cd2+

Received Date:  2020-02-13

Fund Project:  This work was supported by the National Natural Science Foundation of China (Nos. 31671934, 61501295), the Ministry of Science and Technology of China (No. 2017YFC1600603) and the Shanghai Committee of Science and Technology (Nos.18391901200, 17391901500).

设计了一种基于核酸适配体检测镉离子(Cd2+)的电化学生物传感器,将适配体互补链(CDNA)通过AuS键自组装于金电极表面,并与适配体杂交结合形成双链DNA。由于适配体对Cd2+有特异性结合能力,加入Cd2+后,与互补链竞争结合适配体,使修饰二茂铁基团的适配体从金电极表面脱落,二茂铁的电化学信号显著减小。采用方波伏安法(SWV)进行检测,本传感器对Cd2+的线性检测范围为1.0 nmol/L~10.0 μmol/L,检出限为65.1 pmol/L,线性方程为ΔI=0.2872+0.2327lgCR2=0.9972),10 s内即可完成检测。实际江水样品中Cd2+的检测结果与石墨炉原子吸收光谱法的检测结果一致,加标回收率为97.1%~99.5%。本方法灵敏度高、检测速度快、特异性强,在镉环境污染监测方面具有良好的应用前景。

关键词:   镉离子, 电化学, 生物传感器, 适配体, 二茂铁
Key words:   Cadmium ion (II), Electrochemistry, Biosensor, Aptamer, Ferrocene
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一种超灵敏检测镉离子的核酸适配体电化学传感器

袁敏, 钱世权, 曹慧, 徐斐, 叶泰, 于劲松, 郭文, 吴嘉颖, 阿提坎·吾斯曼

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