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引用本文:   谢晓冬, 殷敏, 李茜, 陈楠. 细胞迁移介导的金纳米颗粒外排作用成像研究. 分析化学, 2020, 48(7): 847-854. doi:  10.19756/j.issn.0253-3820.201109 [复制]

Citation:   XIE Xiao-Dong , YIN Min , LI Qian , CHEN Nan . Imaging of Cell Migration Mediated Exocytosis with Gold Nanoprobes. Chinese Journal of Analytical Chemistry, 2020, 48(7): 847-854. doi: 10.19756/j.issn.0253-3820.201109 [复制]

细胞迁移介导的金纳米颗粒外排作用成像研究

通讯作者:  陈楠, nchen@shnu.edu.cn

收稿日期: 2020-03-04

基金项目: 本文系国家自然科学基金项目(Nos.31771102,21974089,91953016)资助

Imaging of Cell Migration Mediated Exocytosis with Gold Nanoprobes

Corresponding author:  CHEN Nan , nchen@shnu.edu.cn

Received Date:  2020-03-04

Fund Project:  This work was supported by the National Natural Science Foundation of China (Nos. 31771102, 21974089, 91953016).

阐明纳米材料的细胞外排途径对于其在临床医疗方面的应用转化至关重要。然而,对于纳米材料是否会在细胞迁移的过程中被清除,还缺乏直接的证据和结论。本研究以金纳米颗粒(AuNPs)作为成像探针,利用暗场显微镜实时地观察和分析了细胞迁移过程中,AuNPs在收缩丝内的运动和外排过程。结果表明,部分被细胞摄取的AuNPs存在于收缩丝内,且颗粒的运动速率随着其与胞体距离的增大而减小,当收缩丝与胞体断开联系后,AuNPs被遗留在胞外。研究结果表明,外源性的纳米材料可以在细胞迁移过程中被外排。此研究结果有助于设计更安全高效的纳米材料,并应用于诊断和治疗。

关键词:   金纳米颗粒, 暗场成像, 细胞迁移, 外排作用
Key words:   Gold nanoparticles, Dark field imaging, Cell migration, Exocytosis
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细胞迁移介导的金纳米颗粒外排作用成像研究

谢晓冬, 殷敏, 李茜, 陈楠

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