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引用本文:   朱超, 赵新颖, 杨歌, 屈锋. 毛细管电泳与核酸适配体高效筛选. 分析化学, 2020, 48(5): 583-589. doi:  10.19756/j.issn.0253-3820.201173 [复制]

Citation:   ZHU Chao , ZHAO Xin-Ying , YANG Ge , QU Feng . Capillary Electrophoresis Involving in High Efficiency Screening for Aptamers. Chinese Journal of Analytical Chemistry, 2020, 48(5): 583-589. doi: 10.19756/j.issn.0253-3820.201173 [复制]

毛细管电泳与核酸适配体高效筛选

收稿日期: 2020-03-31

基金项目: 本文系国家自然科学基金项目(Nos.20875009,21175011,21375008,21675012,21874010,21827810)和北京市科学技术协会2020-2022年度青年人才托举工程项目资助

Capillary Electrophoresis Involving in High Efficiency Screening for Aptamers

Received Date:  2020-03-31

Fund Project:  This work was supported by the National Natural Science Foundation of China (Nos. 20875009, 21175011, 21375008, 21675012, 21874010, 21827810) and the Young Elite Scientist Sponsorship Program of Beijing Association for Science and Technology (2020-2022).

指数富集配体系统进化(SELEX)技术是核酸适配体筛选的通用技术,精准高效的筛选方法和筛选策略设计是成功筛选适配体的关键。本文概述了本课题组自2007年以来,应用毛细管电泳(CE)技术研究核酸适配体高效筛选的有关工作,包括CE在核酸适配体筛选前、筛选中及筛选后各环节中的分析分离应用、不同蛋白靶的分类及筛选策略、多种CE筛选模式和多尺度靶标的核酸适配体筛选。同时,探讨了SELEX中存在的问题,并对其发展前景进行了展望。

关键词:   毛细管电泳, 核酸适配体筛选策略, 多模式筛选, 多尺度靶标, 评述
Key words:   Capillary Electrophoresis, Screening strategy for aptamers, Multiple modes screening, Multi-scale targets, Review
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毛细管电泳与核酸适配体高效筛选

朱超, 赵新颖, 杨歌, 屈锋

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