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引用本文:   曾瑞迪, 杨通, 陈佳汇, 王丹丹, 周小顺, 邵勇. 解聚集诱导发射探针用于人端粒i-Motif二聚体与单体的选择性识别. 分析化学, 2021, 49(6): 992-998. doi:  10.19756/j.issn.0253-3820.210444 [复制]

Citation:   ZENG Rui-Di , YANG Tong , CHEN Jia-Hui , WANG Dan-Dan , ZHOU Xiao-Shun , SHAO Yong . Selective Recognition of Human Telomeric i-Motif Dimer Over Monomer Using A Disaggregation-Induced Emissive Probe. Chinese Journal of Analytical Chemistry, 2021, 49(6): 992-998. doi: 10.19756/j.issn.0253-3820.210444 [复制]

解聚集诱导发射探针用于人端粒i-Motif二聚体与单体的选择性识别

通讯作者:  邵勇, yshao@zjnu.cn

收稿日期: 2021-04-16

基金项目: 浙江省自然科学基金重点项目(No.LZ20B050001)、浙江省万人计划项目(No.2020R52022)、国家自然科学基金项目(No.21675142)、国家级大学生创新创业计划项目(No.201910345027)和浙江师范大学自主设计科研项目(No.2020ZS0304)资助。

Selective Recognition of Human Telomeric i-Motif Dimer Over Monomer Using A Disaggregation-Induced Emissive Probe

Corresponding author:  SHAO Yong , yshao@zjnu.cn

Received Date:  2021-04-16

Fund Project:  Supported by the Key Project of Natural Science Foundation of Zhejiang Province (No.LZ20B050001), the Leading Talent Program of Science and Technology Innovation in Zhejiang (No.2020R52022), the National Natural Science Foundation of China (No.21675142), the National Undergraduate Training Program for Innovation and Entrepreneurship (No.201910345027), and the Independent Designing Scientific Research Project of Zhejiang Normal University (No.2020ZS0304).

由于具有重要的生物活性,并在生物传感器和材料化学等领域广泛应用,i-motif结构开关及识别引起越来越多的关注。分子内i-motif多聚体具有多个重复结构单元,其与i-motif单体的周围环境存在差异。虽然有关i-motif单体的探针识别已有报道,但i-motif多聚体相对于单体的探针选择性识别仍是亟待解决的问题。本研究发现,金丝桃素(Hyp)能选择性区分人端粒分子内i-motif二聚体与单体。二聚体的结合使Hyp的非荧光聚集体发生解聚,产生Turn-on荧光响应,然而Hyp的结合对二聚体结构的稳定性及构象无影响。链接体(Linker)长度实验表明,二聚体中两个i-motif结构单元间的空间可能为Hyp的结合位点,Hyp通过完全氢键互补作用与Loop中的胸腺嘧啶发生结合作用。此结合空间只有在酸性条件下形成i-motif二聚体结构时才能有效结合Hyp。本研究构建的Hyp荧光探针在i-motif多聚体的识别研究中具有重要的作用。

关键词:   分子内i-motif二聚体, i-motif单体, 金丝桃素, 荧光探针, 选择性识别
Key words:   Intramolecular i-motif dimer, i-Motif monomer, Hypericin, Fluorescent probe, Selective recognition
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目录

解聚集诱导发射探针用于人端粒i-Motif二聚体与单体的选择性识别

曾瑞迪, 杨通, 陈佳汇, 王丹丹, 周小顺, 邵勇

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