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引用本文:   陈欢, 韩鑫涛, 魏佳, 刘嘉男, 王国庆, 刘万建, 田永帅, 孟宪瑛, 王振新. 循环肿瘤DNA检测研究新进展. 分析化学, 2021, 49(6): 893-906. doi:  10.19756/j.issn.0253-3820.211106 [复制]

Citation:   CHEN Huan , HAN Xin-Tao , WEI Jia , LIU Jia-Nan , WANG Guo-Qing , LIU Wan-Jian , TIAN Yong-Shuai , MENG Xian-Ying , WANG Zhen-Xin . Recent Progress of Detection of Circulating Tumor DNA. Chinese Journal of Analytical Chemistry, 2021, 49(6): 893-906. doi: 10.19756/j.issn.0253-3820.211106 [复制]

循环肿瘤DNA检测研究新进展

通讯作者:  田永帅, tian_yongshuai@163.com; 孟宪瑛, mengxiany@jlu.edu.cn; 王振新, wangzx@ciac.ac.cn

收稿日期: 2021-02-04

基金项目: 吉林省自然科学基金(学科布局项目)(No.20200201357JC)和山东省泰山产业领军人才工程项目(No.2019TSCYCX-24)资助。

Recent Progress of Detection of Circulating Tumor DNA

Corresponding author:  TIAN Yong-Shuai , tian_yongshuai@163.com; MENG Xian-Ying , mengxiany@jlu.edu.cn; WANG Zhen-Xin , wangzx@ciac.ac.cn

Received Date:  2021-02-04

Fund Project:  Supported by the Natural Science Foundation of Jilin Province (No.20200201357JC) and the Shandong Taishan Industry Leading Talent Project, China (No.2019TSCYCX-24).

循环肿瘤DNA (Circulating tumor DNA,ctDNA)是源自于肿瘤的一种无细胞DNA (Cell free DNA,cfDNA),可能携带与原发肿瘤相同的致癌突变和基因改变。因此,ctDNA有望作为癌症的无创监测生物标志物。研发ctDNA分析新方法可促进用于癌症诊断、预后评估、疗效监测和遗传/表观遗传异常研究的液体活检技术的发展。在过去的几十年中,基于聚合酶链反应(Polymerase chain reaction,PCR)、二代测序(Next-generation sequencing,NGS)等传统的DNA检测技术已被广泛应用于临床样本中ctDNA检测。近来,研究者针对传统DNA检测技术灵敏度较低的问题,研发了多种高性能ctDNA生物传感器用于分析癌症患者血液样本中的ctDNA,为其高灵敏检测提供了新的技术手段。本文综述了ctDNA的分析方法,尤其是近五年来ctDNA生物传感器研究的新进展,探讨了其进入实际临床应用需要解决的技术挑战,为研发癌症液体活检新方法提供了参考。

关键词:   循环肿瘤DNA, 分子标志物, 液体活检, 生物传感器, 评述
Key words:   Circulating tumor DNA, Molecular biomarkers, Liquid biopsy, Biosensor, Review
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循环肿瘤DNA检测研究新进展

陈欢, 韩鑫涛, 魏佳, 刘嘉男, 王国庆, 刘万建, 田永帅, 孟宪瑛, 王振新

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