Citation: Lu Yongzhi , Wang Zhijia . A Modified Derivatization Method for Analysis of Fatty Acids in Eatable Vegetable Oil by Gas Chromatography. Chinese Journal of Analytical Chemistry, 1998, 26(10): 1226-1229. [复制]
A Modified Derivatization Method for Analysis of Fatty Acids in Eatable Vegetable Oil by Gas Chromatography
A modified derivatization method for fatty acids in the eatable vegetable oils was described. When tetramethyl ammonium hydroxide(TMAH) in methanol solution and idomethane (CH3I) were added to the eatable vegetable oils at room temperature, fatty acids in the oils were methylated. Seperation and analysis of various methyl esters of faty acids were performed successfully by using superox-4(25 m×0.2 mm i. d.) F. Q capillary column. The effect of different reaction time and different contents of methylating agent on the degree of methylation were investigated, the optimum derivatization conditions have been established. Composition and content of fatty acids in soybean oil. rapeseed oil, sesameseed oil, salad oil and recovered oil of fried salad oil were determined by this methos.